ArticleJournal of biomedical optics2018
Nondestructive assessment of collagen hydrogel cross-linking using time-resolved autofluorescence imaging.
Article in Journal of biomedical optics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
20 citing papers in PubMed.
- Effects of surgical resection on tissue autofluorescence lifetime signatures in head and neck cancer: implications for intraoperative tumor margin assessment.Journal of biomedical optics · 2026Article
- Article
- Autofluorescence lifetime of gelatin-methacrylate hydrogels is sensitive to changes in crosslinking and post-gelation pH.Frontiers in bioengineering and biotechnology · 2026Article
- Fluorescence saturation imaging microscopy: molecular fingerprinting with a standard confocal microscope.Biomedical optics express · 2024Article
- A Glutaraldehyde-Free Crosslinking Method for the Treatment of Collagen-Based Biomaterials for Clinical Application.Bioengineering (Basel, Switzerland) · 2023Article
- Pre-glycation impairs gelation of high concentration collagen solutions.Journal of biomedical materials research. Part A · 2022Article
- Detection of HOCl-driven degradation of the pericardium scaffolds by label-free multiphoton fluorescence lifetime imaging.Scientific reports · 2022Article
- Use of confocal microscopy imaging for in vitro assessment of adipose-derived mesenchymal stromal cells seeding on acellular dermal matrices: 3D reconstruction based on collagen autofluorescence.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2022Article
- Mechanical performance of collagen gels is dependent on purity, α1/α2 ratio, and telopeptides.Journal of biomedical materials research. Part A · 2022Article
- Skeletal muscle progenitors are sensitive to collagen architectural features of fibril size and cross linking.American journal of physiology. Cell physiology · 2021Article
- Physical, Biomechanical, and Optical Characterization of Collagen and Elastin Blend Hydrogels.Annals of biomedical engineering · 2020Article
- Collagen: quantification, biomechanics, and role of minor subtypes in cartilage.Nature reviews. Materials · 2020Article
- FLIm and Raman Spectroscopy for Investigating Biochemical Changes of Bovine Pericardium upon Genipin Cross-Linking.Molecules (Basel, Switzerland) · 2020Article
- FLIm-Guided Raman Imaging to Study Cross-Linking and Calcification of Bovine Pericardium.Analytical chemistry · 2020Article
- Skin Structure-Function Relationships and the Wound Healing Response to Intrinsic Aging.Advances in wound care · 2020Review
- Simultaneous intraluminal imaging of tissue autofluorescence and eGFP-labeled cells in engineered vascular grafts inside a bioreactor.Methods and applications in fluorescence · 2019Article
- Multiscale, multispectral fluorescence lifetime imaging using a double-clad fiber.Optics letters · 2019Article
- Multimodal Label-Free Imaging for Detecting Maturation of Engineered Osteogenic Grafts.ACS biomaterials science & engineering · 2019Article
- Tissue Engineering: Understanding the Role of Biomaterials and Biophysical Forces on Cell Functionality Through Computational and Structural Biotechnology Analytical Methods.Computational and structural biotechnology journal · 2019Review
- Label-free assessment of carotid artery biochemical composition using fiber-based fluorescence lifetime imaging.Biomedical optics express · 2018Article
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Authors and funding
8 authors.
Funding
Abstract
We investigate the use of a fiber-based, multispectral fluorescence lifetime imaging (FLIm) system to nondestructively monitor changes in mechanical properties of collagen hydrogels caused by controlled application of widely used cross-linking agents, glutaraldehyde (GTA) and ribose. Postcross-linking, fluorescence lifetime images are acquired prior to the hydrogels being processed by rheological or tensile testing to directly probe gel mechanical properties. To preserve the sterility of the ribose-treated gels, FLIm is performed inside a biosafety cabinet (BSC). A pairwise correlation analysis is used to quantify the relationship between mean hydrogel fluorescence lifetimes and the storage or Young's moduli of the gels. In the GTA study, we observe strong and specific correlations between fluorescence lifetime and the storage and Young's moduli. Similar correlations are not observed in the ribose study and we postulate a reason for this. Finally, we demonstrate the ability of FLIm to longitudinally monitor dynamic cross-link formation. The strength of the GTA correlations and deployment of our fiber-based FLIm system inside the aseptic environment of a BSC suggests that this technique may be a valuable tool for the tissue engineering community where longitudinal assessment of tissue construct maturation in vitro is highly desirable.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.