Evidence map›Paper›PMID 29512142›Full record

ArticleCurrent protocols in immunology2018

Liposome Preparation for the Analysis of Lipid-Receptor Interaction and Efferocytosis.

Oliver H Voss, Ha-Na Lee, Linjie Tian, Konrad Krzewski, John E Coligan

Abstract read
In one paragraph

Article in Current protocols in immunology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Oliver H VossReceptor Cell Biology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland.
Ha-Na LeeReceptor Cell Biology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland.
Linjie TianReceptor Cell Biology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland.
Konrad KrzewskiReceptor Cell Biology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland.
John E ColiganReceptor Cell Biology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland.
National Institute of Allergy and Infectious Diseases · USNational Institutes of Health · US

Funding

Role of NKG2 Family Receptors in Regulating the Immune ResponseZ01AI000963 · NIAID · NIAID EXTRAMURAL ACTIVITIES · PI COLIGAN, JOHN E · 2006 to 2008
$1.7M
Enhancing Immunotherapeutic Value of Lymphocytes by Controlling ApoptosisZ01AI000962 · NIAID · NIAID EXTRAMURAL ACTIVITIES · PI COLIGAN, JOHN E · 2006 to 2008
$933k
Intramural NIH HHS Z01 AI000962Intramural NIH HHS Z01 AI000963
6 · The paper itself

Abstract

Efficient phagocytosis of apoptotic cells (efferocytosis) is essential for immune homeostasis. Phospholipids exposed on the surface of apoptotic cells, such as phosphatidylserine, supply important "eat-me" signals. Liposomes are lipid bilayer vesicles that can be generated from one or several types of phospholipids of interest. Thus, these vesicles offer versatility, flexibility, and, importantly, a three-dimensional structure for studying the interaction between lipids and their receptors as well as the lipid-receptor interaction-mediated signaling events controlling efferocytosis by cells like professional phagocytes. Here, we describe methods to prepare liposomes, perform liposome-based lipid-receptor binding assays, use liposomes to block efferocytosis, and utilize liposome-coated beads as apoptotic cell surrogates for phagocytosis. © 2018 by John Wiley & Sons, Inc.

Indexed as

LiposomesPhagocytosisAnimalsApoptosisMice, Inbred C57BLPhospholipidsReceptors, Cell SurfaceSurface Plasmon ResonanceThymocytesLiposomesPhospholipidsReceptors, Cell Surfaceefferocytosislipid-receptor interactionliposome-coated beadsliposomesphospholipids

Identifiers

PMID29512142
PMCPMC5844359
OpenAlexW2790437587

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.