ArticlePlant cell reports2018
Overexpression of VpPR10.1 by an efficient transformation method enhances downy mildew resistance in V. vinifera.
Article in Plant cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- Exploring Agrobacterium-mediated genetic transformation methods and its applications in Lilium.Plant methods · 2024Review
- The Single-Stranded DNA-Binding GeneInternational journal of molecular sciences · 2022Article
- Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola.Horticulture research · 2021Article
- New Technologies and Strategies for Grapevine Breeding Through Genetic Transformation.Frontiers in plant science · 2021Review
- Comprehensive Genomic Analysis of the EndophyticFrontiers in genetics · 2021Article
- Characterization ofFrontiers in microbiology · 2021Article
- Biotechnological Approaches: Gene Overexpression, Gene Silencing, and Genome Editing to Control Fungal and Oomycete Diseases in Grapevine.International journal of molecular sciences · 2020Review
- CRISPR/Cas9-mediatedHorticulture research · 2020Article
- Improving T-DNA Transfer toFrontiers in plant science · 2020Article
- Transcriptomic analysis of Chinese wild Vitis pseudoreticulata in response to Plasmopara viticola.Protoplasma · 2019Article
- Establishment of Efficient Genetic Transformation Systems and Application of CRISPR/Cas9 Genome Editing Technology inInternational journal of molecular sciences · 2019Article
- Genome-wide analysis of glyoxalase-like gene families in grape (Vitis vinifera L.) and their expression profiling in response to downy mildew infection.BMC genomics · 2019Article
- The Nuclear-Localized RxLR Effector PvAvh74 FromFrontiers in microbiology · 2019Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
key messagePutrescine and spermidine increase the transformation efficiency of Vitis vinifera L. cv. Thompson seedless. Accumulation of VpPR10.1 in transgenic V. vinifera Thompson seedless, likely increases its resistance to downy mildew. A more efficient method is described for facilitating Agrobacterium-mediated transformation of Vitis vinifera L. cv. Thompson Seedless somatic embryogenesis using polyamines (PAs). The efficacies of putrescine, spermidine and spermine are identified at a range of concentrations (10 µM, 100 µM and 1 mM) added to the culture medium during somatic embryo growth. Putrescine (PUT) and spermidine (SPD) promote the recovery of proembryonic masses (PEM) and the development of somatic embryos (SE) after co-cultivation. Judging from the importance of the time-frame in genetic transformation, PAs added at the co-cultivation stage have a stronger effect than delayed selection treatments, which are superior to antibiotic treatments in the selection stage. Best embryogenic responses are with 1 mM PUT and 100 µM SPD added to the co-culture medium. Using the above method, a pathogenesis-related gene (VpPR10.1) from Chinese wild Vitis pseudoreticulata was transferred into Thompson Seedless for functional evaluation. The transgenic line, confirmed by western blot analysis, was inoculated with Plasmopara viticola to test for downy mildew resistance. Based on observed restrictions of hyphal growth and increases in H
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