ArticleScientific reports2018
Single-cell RNA sequencing reveals developmental heterogeneity of blastomeres during major genome activation in bovine embryos.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates.Frontiers in veterinary science · 2021Pooled it
- Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy.Scientific reports · 2019Trial
- LivestockDev: A multi-omics resource for exploring cross-species comparison of livestock embryogenesis.Zoological research · 2026Article
- Early transcriptional divergence underlies cell fate bias in bovine embryos.Communications biology · 2026Article
- Species-specific roles of SP1 in bovine and human embryonic genome activation and early embryonic development.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Integrated analysis of DNA methylation and gene expression in dairy cows during early pregnancy.BMC genomics · 2026Article
- Genome-Wide Association Study of Daughter Pregnancy Rate in Crossbred Dairy Cows.International journal of molecular sciences · 2025Article
- RNA sequencing of mRNA 5'-ends reveals regulators of bovine embryonic genome activation.BMC genomics · 2025Article
- Elucidation of the pluripotent potential of bovine embryonic lineages facilitates the establishment of formative stem cell lines.Cellular and molecular life sciences : CMLS · 2024Article
- Identification of novel cattle (Bos taurus) genes and biological insights of their function in pre-implantation embryo development.BMC genomics · 2024Article
- Single-Cell Sequencing Technology in Ruminant Livestock: Challenges and Opportunities.Current issues in molecular biology · 2024Review
- Pleomorphic adenoma gene1 in reproduction and implication for embryonic survival in cattle: a review.Journal of animal science · 2024Review
- Single-cell RNA-seq and single-cell bisulfite-sequencing reveal insights into yak preimplantation embryogenesis.The Journal of biological chemistry · 2024Article
- PDGFRβ Activation Induced the Bovine Embryonic Genome Activation via Enhanced NFYA Nuclear Localization.International journal of molecular sciences · 2023Article
- Molecular and cellular programs underlying the development of bovine pre-implantation embryos.Reproduction, fertility, and development · 2023Article
- Pre-Implantation Bovine Embryo Evaluation-From Optics to Omics and Beyond.Animals : an open access journal from MDPI · 2023Review
- Article
- Bovine blastocyst-like structures derived from stem cell cultures.Cell stem cell · 2023Article
- Reprogramming barriers in bovine cells nuclear transfer revealed by single-cell RNA-seq analysis.Journal of cellular and molecular medicine · 2022Article
- A New Toolbox in Experimental Embryology-Alternative Model Organisms for Studying Preimplantation Development.Journal of developmental biology · 2021Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic development is initially controlled by maternal RNAs and proteins stored in the oocyte, until gene products gradually generated by the embryo itself take over. Major embryonic genome activation (EGA) in bovine embryos occurs at the eight- to 16-cell stage. Morphological observations, such as size of blastomeres and distribution of microvilli, suggested heterogeneity among individual cells already at this developmental stage. To address cell heterogeneity on the transcriptome level, we performed single-cell RNA sequencing of 161 blastomeres from 14 in vitro produced bovine embryos at Day 2 (n = 6) and Day 3 (n = 8) post fertilization. Complementary DNA libraries were prepared using the Single-Cell RNA-Barcoding and Sequencing protocol and sequenced. Non-supervised clustering of single-cell transcriptome profiles identified six clusters with specific sets of genes. Most embryos were comprised of cells from at least two different clusters. Sorting cells according to their transcriptome profiles resulted in a non-branched pseudo-time line, arguing against major lineage inclination events at this developmental stage. In summary, our study revealed heterogeneity of transcriptome profiles among single cells in bovine Day 2 and Day 3 embryos, suggesting asynchronous blastomere development during the phase of major EGA.
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Registered trials
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