ArticleScientific reports2018
Direct Detection of Unamplified Pathogen RNA in Blood Lysate using an Integrated Lab-in-a-Stick Device and Ultrabright SERS Nanorattles.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 60 citations in OpenAlex.
- Amplification of RNA for identification of Zika and HCV in whole blood.Science advances · 2026Article
- Picogram-level detection of endotoxin in human saliva using ultrasensitive surface enhanced Raman scattering in lateral flow assay devices.RSC advances · 2025Article
- Plasmonics-enhanced spikey nanorattle-based biosensor for direct SERS detection of mRNA cancer biomarkers.Analytical and bioanalytical chemistry · 2024Article
- Nanoplasmonics biosensors: At the frontiers of biomedical diagnostics.Trends in analytical chemistry : TRAC · 2024Article
- Dual-Modal Colorimetric and Surface-Enhanced Raman Scattering (SERS)-Based Lateral Flow Immunoassay for Ultrasensitive Detection of SARS-CoV-2 Using a Plasmonic Gold Nanocrown.Analytical chemistry · 2024Article
- Machine learning using convolutional neural networks for SERS analysis of biomarkers in medical diagnostics.Journal of Raman spectroscopy : JRS · 2022Article
- Recent Advances Towards Point-Of-Care Applications of Surface-Enhanced Raman Scattering Sensing.Frontiers in chemistry · 2021Review
- Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases.Biosensors & bioelectronics · 2020Review
- Plasmonic nanobiosensors for detection of microRNA cancer biomarkers in clinical samples.The Analyst · 2020Article
- Present and Future of Surface-Enhanced Raman Scattering.ACS nano · 2020Article
- Application of Vibrational Spectroscopy and Imaging to Point-of-Care Medicine: A Review.Applied spectroscopy · 2018Review
- Rapid Nanophotonics Assay for Head and Neck Cancer Diagnosis.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Direct detection of genetic biomarkers in body fluid lysate without target amplification will revolutionize nucleic acid-based diagnostics. However, the low concentration of target sequences makes this goal challenging. We report a method for direct detection of pathogen RNA in blood lysate using a bioassay using surface-enhanced Raman spectroscopy (SERS)-based detection integrated in a "lab-in-a-stick" portable device. Two levels of signal enhancement were employed to achieve the sensitivity required for direct detection. Each target sequence was tagged with an ultrabright SERS-encoded nanorattle with ultrahigh SERS signals, and these tagged target sequences were concentrated into a focused spot for detection using hybridization sandwiches with magnetic microbeads. Furthermore, the washing process was automated by integration into a "lab-in-a-stick" portable device. We could directly detect synthetic target with a limit of detection of 200 fM. More importantly, we detected plasmodium falciparum malaria parasite RNA directly in infected red blood cells lysate. To our knowledge, this is the first report of SERS-based direct detection of pathogen nucleic acid in blood lysate without nucleic acid extraction or target amplification. The results show the potential of our integrated bioassay for field use and point-of-care diagnostics.
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Registered trials
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