ArticleParasites & vectors2018
A recombinant Fasciola gigantica 14-3-3 epsilon protein (rFg14-3-3e) modulates various functions of goat peripheral blood mononuclear cells.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Immune modulation of buffalo peripheral blood mononuclear cells by two asparaginyl endopeptidases from Fasciola gigantica.Parasites & vectors · 2024Article
- Reconstruction of the TGF-β signaling pathway of Fasciola gigantica.Parasitology research · 2023Review
- Proteomic analysis of exosome-like vesicles from Fasciola gigantica adult worm provides support for new vaccine targets against fascioliasis.Parasites & vectors · 2023Article
- The 22nd Chromatography Component of theAnimals : an open access journal from MDPI · 2023Article
- Proteomic analysis of the excretory-secretory products from Strongyloides venezuelensis infective larvae: new insights for the immunodiagnosis of human strongyloidiasis.Parasitology research · 2022Article
- Molecular Characteristics and Potent Immunomodulatory Activity of Fasciola hepatica Cystatin.The Korean journal of parasitology · 2022Article
- A global phosphoproteomics analysis of adult Fasciola gigantica by LC-MS/MS.Parasitology research · 2022Article
- Proteomic analysis ofFrontiers in microbiology · 2022Article
- Proteomic and Immunological Identification of Diagnostic Antigens from Spirometra erinaceieuropaei Plerocercoid.The Korean journal of parasitology · 2021Article
- Current Status for Controlling the Overlooked Caprine Fasciolosis.Animals : an open access journal from MDPI · 2021Review
- YWHAE as an HE4 interacting protein can influence the malignant behaviour of ovarian cancer by regulating the PI3K/AKT and MAPK pathways.Cancer cell international · 2021Article
- Fasciola gigantica tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes.Parasites & vectors · 2021Article
- Article
- Proteomic Profiling of the Liver, Hepatic Lymph Nodes, and Spleen of Buffaloes Infected withPathogens (Basel, Switzerland) · 2020Article
- Unveiling the Immunomodulatory Characteristics ofAnimals : an open access journal from MDPI · 2020Article
- Modulation of the Functions of Goat Peripheral Blood Mononuclear Cells byPathogens (Basel, Switzerland) · 2020Article
- HcTTR: a novel antagonist against goat interleukin 4 derived from the excretory and secretory products of Haemonchus contortus.Veterinary research · 2019Article
- The MultitaskingFrontiers in immunology · 2019Article
- 14-3-3ζ-A Novel Immunogen Promotes Inflammatory Cytokine Production.Frontiers in immunology · 2019Article
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundThe molecular structure of Fasciola gigantica 14-3-3 protein has been characterized. However, the involvement of this protein in parasite pathogenesis remains elusive and its effect on the functions of innate immune cells is unknown. We report on the cloning and expression of a recombinant F. gigantica 14-3-3 epsilon protein (rFg14-3-3e), and testing its effects on specific functions of goat peripheral blood mononuclear cells (PBMCs).
methodsrFg14-3-3e protein was expressed in Pichia pastoris. Western blot and immunofluorescence assay (IFA) were used to examine the reactivity of rFg14-3-3e protein to anti-F. gigantica and anti-rFg14-3-3e antibodies, respectively. Various assays were used to investigate the stimulatory effects of the purified rFg14-3-3e protein on specific functions of goat PBMCs, including cytokine secretion, proliferation, migration, nitric oxide (NO) production, phagocytosis, and apoptotic capabilities. Potential protein interactors of rFg14-3-3e were identified by querying the databases Intact, String, BioPlex and BioGrid. A Total Energy analysis of each of the identified interaction was performed. Gene Ontology (GO) enrichment analysis was conducted using Funcassociate 3.0.
resultsSequence analysis revealed that rFg14-3-3e protein had 100% identity to 14-3-3 protein from Fasciola hepatica. Western blot analysis showed that rFg14-3-3e protein is recognized by sera from goats experimentally infected with F. gigantica and immunofluorescence staining using rat anti-rFg14-3-3e antibodies demonstrated the specific binding of rFg14-3-3e protein to the surface of goat PBMCs. rFg14-3-3e protein stimulated goat PBMCs to produce interleukin-10 (IL-10) and transforming growth factor beta (TGF-β), corresponding with low levels of IL-4 and interferon gamma (IFN-γ). Also, this recombinant protein promoted the release of NO and cell apoptosis, and inhibited the proliferation and migration of goat PBMCs and suppressed monocyte phagocytosis. Homology modelling revealed 65% identity between rFg14-3-3e and human 14-3-3 protein YWHAE. GO enrichment analysis of the interacting proteins identified terms related to apoptosis, protein binding, locomotion, hippo signalling and leukocyte and lymphocyte differentiation, supporting the experimental findings.
conclusionsOur data suggest that rFg14-3-3e protein can influence various cellular and immunological functions of goat PBMCs in vitro and may be involved in mediating F. gigantica pathogenesis. Because of its involvement in F. gigantica recognition by innate immune cells, rFg14-3-3e protein may have applications for development of diagnostics and therapeutic interventions.
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