ArticleSensors (Basel, Switzerland)2018
A New Method for Immobilization of His-Tagged Proteins with the Application of Low-Frequency AC Electric Field.
Article in Sensors (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Research progress of multi-enzyme complexes based on the design of scaffold protein.Bioresources and bioprocessing · 2023Review
- Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials.Journal of advanced research · 2022Review
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Authors and funding
7 authors.
Funding
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Abstract
Continued advancement of protein array, bioelectrode, and biosensor technologies is necessary to develop methods for higher amount and highly oriented immobilization activity of proteins. In pursuit of these goals, we developed a new immobilization method by combining electrostatic transport and subsequent molecular diffusion of protein molecules. Our developed immobilization method is based on a model that transports proteins toward the substrate surface due to steep concentration gradient generated by low-frequency AC electric field. The immobilization of the maximum amounts can be obtained by the application of the AC voltage of 80 Vpp, 20 Hz both for His-tagged Green Fluorescent Protein (GFP) and
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