Evidence map›Paper›PMID 29510577›Full record

ArticleViruses2018

Loss of Actin-Based Motility Impairs Ectromelia Virus Release In Vitro but Is Not Critical to Spread In Vivo.

Melanie Laura Duncan, Jacquelyn Horsington, Preethi Eldi, Zahrah Al Rumaih, Gunasegaran Karupiah, Timothy P Newsome

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Identifying potential monkeypox virus inhibitors: anFrontiers in cellular and infection microbiology · 2024
    Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Melanie Laura DuncanSchool of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia. melanie.duncan@sydney.edu.au.
Jacquelyn HorsingtonSchool of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia. jacquelynjh@gmail.com.
Preethi EldiSchool of Medicine, College of Health and Medicine, The University of Tasmania, Hobart, TAS 7005, Australia. preethi.eldi@gmail.com.
Zahrah Al RumaihSchool of Medicine, College of Health and Medicine, The University of Tasmania, Hobart, TAS 7005, Australia. rumaihxj@gmail.com.
Gunasegaran KarupiahSchool of Medicine, College of Health and Medicine, The University of Tasmania, Hobart, TAS 7005, Australia. guna.karupiah@utas.edu.au.
Timothy P NewsomeSchool of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia. tim.newsome@sydney.edu.au.ORCID 0000-0002-2193-596X
Australian National University · AUThe University of Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ectromelia virus (ECTV) is an orthopoxvirus and the causative agent of mousepox. Like other poxviruses such as variola virus (agent of smallpox), monkeypox virus and vaccinia virus (the live vaccine for smallpox), ECTV promotes actin-nucleation at the surface of infected cells during virus release. Homologs of the viral protein A36 mediate this function through phosphorylation of one or two tyrosine residues that ultimately recruit the cellular Arp2/3 actin-nucleating complex. A36 also functions in the intracellular trafficking of virus mediated by kinesin-1. Here, we describe the generation of a recombinant ECTV that is specifically disrupted in actin-based motility allowing us to examine the role of this transport step in vivo for the first time. We show that actin-based motility has a critical role in promoting the release of virus from infected cells in vitro but plays a minor role in virus spread in vivo. It is likely that loss of microtubule-dependent transport is a major factor for the attenuation observed when

Indexed as

Virus ReleaseActinsAnimalsBiological TransportCell LineChlorocebus aethiopsEctromelia, InfectiousEctromelia virusGene ExpressionHumansMiceMice, KnockoutMutationProtein BindingViral ProteinsActinsViral ProteinsA36actin-based motilityArp2/3 complexviral releasevirus transport

Identifiers

PMID29510577
PMCPMC5869504
OpenAlexW2791280628

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.