ArticlePlant biotechnology journal2018
The rice terpene synthase gene OsTPS19 functions as an (S)-limonene synthase in planta, and its overexpression leads to enhanced resistance to the blast fungus Magnaporthe oryzae.
Article in Plant biotechnology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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53 citing papers in PubMed, 112 citations in OpenAlex.
- Molecular insights into Valencene synthase (SgTPS-V) and its role in sesquiterpenoid biosynthesis.BMC plant biology · 2026Article
- RING-Type E3 Ligase OsRFPH2-6 Ubiquitinates Nuclear Factor Y Subunit OsNF-YA1 to Suppress Rice Immunity.Molecular plant pathology · 2025Article
- Genome-wide analysis of terpene synthase family in peanut (Arachis hypogaea L.) explores the potential cross-talk in terpenoid biosynthesis.BMC genomics · 2025Article
- Integrative Transcriptomic and Metabolomic Analyses Elucidate the Molecular Mechanisms Underlying Enhanced Yield and Bacterial Blight Resistance in the RXN2 Rice Cultivar.Plants (Basel, Switzerland) · 2025Article
- Unveiling molecular mechanisms and candidate genes for goss's bacterial wilt and leaf blight resistance in corn through RNA-Seq analysis.BMC genomics · 2025Article
- Utilizing plant synthetic biology to accelerate plant-microbe interactions research.Biodesign research · 2025Review
- Limonene enhances rice plant resistance to a piercing-sucking herbivore and rice pathogens.Plant biotechnology journal · 2025Article
- Expansion and functional divergence of terpene synthase genes in angiosperms: a driving force of terpene diversity.Horticulture research · 2025Article
- Transcriptome profiles reveal response mechanisms and key role of PsNAC1 in Pinus sylvestris var. mongolica to drought stress.BMC plant biology · 2024Article
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- The reproductive strategy of a typical distylousFrontiers in plant science · 2024Article
- Chemical Structure Diversity and Extensive Biological Functions of Specialized Metabolites in Rice.International journal of molecular sciences · 2023Review
- Integration of transcriptome and metabolome analyses reveals the role of OsSPL10 in rice defense against brown planthopper.Plant cell reports · 2023Article
- Secondary Metabolites and Their Role in Strawberry Defense.Plants (Basel, Switzerland) · 2023Review
- Article
- Advances in the Biosynthesis of Terpenoids and Their Ecological Functions in Plant Resistance.International journal of molecular sciences · 2023Review
- Allelic variation of terpene synthases drives terpene diversity in the wild species of the Freesia genus.Plant physiology · 2023Article
- The potential role of plant secondary metabolites on antifungal and immunomodulatory effect.Applied microbiology and biotechnology · 2023Review
- Selection and Validation of Optimal RT-qPCR Reference Genes for the Normalization of Gene Expression under Different Experimental Conditions inPlants (Basel, Switzerland) · 2023Article
- Genome-Wide Association Study Identifies Resistance Loci for Bacterial Blight in a Collection of Asian TemperateInternational journal of molecular sciences · 2023Article
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Authors and funding
13 authors at 6 institutions in 3 countries.
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Abstract
Rice blast disease, caused by the fungus Magnaporthe oryzae, is the most devastating disease of rice. In our ongoing characterization of the defence mechanisms of rice plants against M. oryzae, a terpene synthase gene OsTPS19 was identified as a candidate defence gene. Here, we report the functional characterization of OsTPS19, which is up-regulated by M. oryzae infection. Overexpression of OsTPS19 in rice plants enhanced resistance against M. oryzae, while OsTPS19 RNAi lines were more susceptible to the pathogen. Metabolic analysis revealed that the production of a monoterpene (S)-limonene was increased and decreased in OsTPS19 overexpression and RNAi lines, respectively, suggesting that OsTPS19 functions as a limonene synthase in planta. This notion was further supported by in vitro enzyme assays with recombinant OsTPS19, in which OsTPS19 had both sesquiterpene activity and monoterpene synthase activity, with limonene as a major product. Furthermore, in a subcellular localization experiment, OsTPS19 was localized in plastids. OsTPS19 has a highly homologous paralog, OsTPS20, which likely resulted from a recent gene duplication event. We found that the variation in OsTPS19 and OsTPS20 enzyme activities was determined by a single amino acid in the active site cavity. The expression of OsTPS20 was not affected by M. oryzae infection. This indicates functional divergence of OsTPS19 and OsTPS20. Lastly, (S)-limonene inhibited the germination of M. oryzae spores in vitro. OsTPS19 was determined to function as an (S)-limonene synthase in rice and plays a role in defence against M. oryzae, at least partly, by inhibiting spore germination.
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