ArticlePloS one2018
Reversible and long-term immobilization in a hydrogel-microbead matrix for high-resolution imaging of Caenorhabditis elegans and other small organisms.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Hydrogels in medicine and biotechnology.Scientific reports · 2026Article
- A Real-Time Automated Deep Learning Workflow for Non-invasive High-Magnification Imaging ofbioRxiv : the preprint server for biology · 2026Article
- SIN-3 coregulator maintains adaptive capacity to different diets in Caenorhabditis elegans through vitamin B12.G3 (Bethesda, Md.) · 2026Article
- Metabolites from chia seed (Salvia Hispanica L.) exert antioxidant effects through activation of the DAF-16 pathway and neuroprotective activity in the Caenorhabditis elegans model of Huntington's disease.BMC complementary medicine and therapies · 2026Article
- Enabling global access to potent subunit vaccines with a simple and scalable injectable hydrogel platform.Biomaterials science · 2026Article
- Visualization and Analyses of Cytoplasmic Streaming in C. elegans Zygotes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Quinic acid protects against the development of Huntington's disease in Caenorhabditis elegans model.BMC complementary medicine and therapies · 2024Article
- Review
- SMORES: a simple microfluidic operating room for the examination and surgery of Stentor coeruleus.Scientific reports · 2024Article
- SMORES: A Simple Microfluidic Operating Room for the Examination and Surgery ofbioRxiv : the preprint server for biology · 2024Article
- Alginate Gel Immobilization ofBio-protocol · 2023Article
- Microemulsions as Lipid Nanosystems Loaded into Thermoresponsive In Situ Microgels for Local Ocular Delivery of Prednisolone.Pharmaceutics · 2022Article
- Assessment of dopaminergic neuron degeneration in aSTAR protocols · 2022Article
- Observing and Quantifying Fluorescent Reporters.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Imaging of Actin Cytoskeleton in the Nematode Caenorhabditis elegans.Methods in molecular biology (Clifton, N.J.) · 2022Article
- CentTracker: a trainable, machine-learning-based tool for large-scale analyses ofMolecular biology of the cell · 2021Article
- Integrator is recruited to promoter-proximally paused RNA Pol II to generate Caenorhabditis elegans piRNA precursors.The EMBO journal · 2021Article
- Biophysical analysis of drug efficacy on C. elegans models for neurodegenerative and neuromuscular diseases.PloS one · 2021Article
- Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans.Journal of visualized experiments : JoVE · 2020Article
- Surveillance of cohesin-supported chromosome structure controls meiotic progression.Nature communications · 2020Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nematode Caenorhabditis elegans is an important model organism for biomedical research and genetic studies relevant to human biology and disease. Such studies are often based on high-resolution imaging of dynamic biological processes in the worm body tissues, requiring well-immobilized and physiologically active animals in order to avoid movement-related artifacts and to obtain meaningful biological information. However, existing immobilization methods employ the application of either anesthetics or servere physical constraints, by using glue or specific microfluidic on-chip mechanical structures, which in some cases may strongly affect physiological processes of the animals. Here, we immobilize C. elegans nematodes by taking advantage of a biocompatible and temperature-responsive hydrogel-microbead matrix. Our gel-based immobilization technique does not require a specific chip design and enables fast and reversible immobilization, thereby allowing successive imaging of the same single worm or of small worm populations at all development stages for several days. We successfully demonstrated the applicability of this method in challenging worm imaging contexts, in particular by applying it for high-resolution confocal imaging of the mitochondrial morphology in worm body wall muscle cells and for the long-term quantification of number and size of specific protein aggregates in different C. elegans neurodegenerative disease models. Our approach was also suitable for immobilizing other small organisms, such as the larvae of the fruit fly Drosophila melanogaster and the unicellular parasite Trypanosoma brucei. We anticipate that this versatile technique will significantly simplify biological assay-based longitudinal studies and long-term observation of small model organisms.
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