ArticleScientific reports2018
Three dimensional electron microscopy reveals changing axonal and myelin morphology along normal and partially injured optic nerves.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed.
- Label-free tracking of subcortical white matter degradationBiomedical optics express · 2026Article
- Beyond Organ Boundaries: Molecular Mechanisms of Hepatic Encephalopathy and Parkinson's Disease from the Perspective of the Gut-Liver-Brain Axis.Research (Washington, D.C.) · 2026Review
- MyelinASN neuro · 2026Article
- Where Is the Oxygen? The Mirage of Non-Oxidative Glucose Consumption During Brain Activity.NeuroSci · 2025Review
- Light-evoked activity and BDNF regulate mitochondrial dynamics and mitochondrial localized translation in CNS axons.iScience · 2025Article
- Demystifying The MyelinASN neuro · 2025Article
- Cortical white matter: no longer a silent partner.Frontiers in neuroanatomy · 2025Review
- Remote Ischemic Post-Conditioning (RIC) Mediates Anti-Inflammatory Signaling via Myeloid AMPKα1 in Murine Traumatic Optic Neuropathy (TON).International journal of molecular sciences · 2024Article
- NerveTracker: a Python-based software toolkit for visualizing and tracking groups of nerve fibers in serial block-face microscopy with ultraviolet surface excitation images.Journal of biomedical optics · 2024Article
- Disruption of myelin structure and oligodendrocyte maturation in a macaque model of congenital Zika infection.Nature communications · 2024Article
- Sample preparation and data collection for serial block face scanning electron microscopy of mammalian cell monolayers.PloS one · 2024Article
- Microglia/macrophages are ultrastructurally altered by their proximity to spinal cord injury in adult female mice.Journal of neuroinflammation · 2023Article
- Serial Block Face-Scanning Electron Microscopy as a Burgeoning Technology.Advanced biology · 2023Review
- Secondary Degeneration Impairs Myelin Ultrastructural Development in Adulthood following Adolescent Neurotrauma in the Rat Optic Nerve.International journal of molecular sciences · 2023Article
- Modeling Reactive Oxygen Species-Induced Axonal Loss in Leber Hereditary Optic Neuropathy.Biomolecules · 2022Article
- The Mechanical Microenvironment Regulates Axon Diameters Visualized by Cryo-Electron Tomography.Cells · 2022Article
- Article
- Targeted neuromodulation of pelvic floor nerves in aging and multiparous rabbits improves continence.Scientific reports · 2021Article
- Autofluorescence enhancement for label-free imaging of myelinated fibers in mammalian brains.Scientific reports · 2021Article
- A shape-adjusted ellipse approach corrects for varied axonal dispersion angles and myelination in primate nerve roots.Scientific reports · 2021Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Following injury to the central nervous system, axons and myelin distinct from the initial injury site undergo changes associated with compromised function. Quantifying such changes is important to understanding the pathophysiology of neurotrauma; however, most studies to date used 2 dimensional (D) electron microscopy to analyse single sections, thereby failing to capture changes along individual axons. We used serial block face scanning electron microscopy (SBF SEM) to undertake 3D reconstruction of axons and myelin, analysing optic nerves from normal uninjured female rats and following partial optic nerve transection. Measures of axon and myelin dimensions were generated by examining 2D images at 5 µm intervals along the 100 µm segments. In both normal and injured animals, changes in axonal diameter, myelin thickness, fiber diameter, G-ratio and percentage myelin decompaction were apparent along the lengths of axons to varying degrees. The range of values for axon diameter along individual reconstructed axons in 3D was similar to the range from 2D datasets, encompassing reported variation in axonal diameter attributed to retinal ganglion cell diversity. 3D electron microscopy analyses have provided the means to demonstrate substantial variability in ultrastructure along the length of individual axons and to improve understanding of the pathophysiology of neurotrauma.
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