ArticleNature neuroscience2018
Nontoxic, double-deletion-mutant rabies viral vectors for retrograde targeting of projection neurons.
Article in Nature neuroscience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
113 citing papers in PubMed, 193 citations in OpenAlex.
- Viral Tracing Gone Viral: Expanded Possibilities Call for Increased Caution in Describing Cerebellar Network Connections.Cerebellum (London, England) · 2026Review
- An applicable and efficient retrograde monosynaptic circuit mapping tool for larval zebrafish.eLife · 2026Article
- TRACR: an anterograde transneuronal tracing system for genetic access across synapses and longitudinal circuit analysis.bioRxiv : the preprint server for biology · 2026Article
- Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas.Nature protocols · 2026Review
- Engineering multiple levels of specificity in an RNA viral vector.Nature communications · 2026Article
- Preparation and application of YFV-17D-derived Anterograde Trans-neuronal Viral Vectors for Neuroscience Research.bioRxiv : the preprint server for biology · 2026Article
- SpaGene: A Deep Adversarial Framework for Spatial Gene Imputation.Computational and structural biotechnology journal · 2026Article
- Transsynaptic viral tools in neural circuit analysis: from anatomical mapping to functional interrogation.Frontiers in neural circuits · 2026Review
- Dissecting mammalian cortical circuit development at single-cell resolution using inducible barcoded rabies virus.bioRxiv : the preprint server for biology · 2025Article
- Hippocampal neural circuit mechanisms in Alzheimer's disease revealed by viral-genetic circuit mapping.Neurobiology of disease · 2025Review
- Risk reshapes amygdala representation of choice.bioRxiv : the preprint server for biology · 2025Article
- SpaGene: A Deep Adversarial Framework for Spatial Gene Imputation.bioRxiv : the preprint server for biology · 2025Article
- Neural networks of the mouse visceromotor cortex.Nature · 2025Article
- Green-to-red spectral labeling: A novel polysynaptic retrograde tracing strategy in the marker footprint mouse model.Animal models and experimental medicine · 2025Article
- Development of Cre-dependent retrograde trans-multisynaptic tracer based on pseudorabies virus bartha strain.Molecular brain · 2025Article
- Layer-Specific Connectivity and Functional Interference of Chrna2+ Layer 5 Martinotti Cells in the Primary Motor Cortex.The European journal of neuroscience · 2025Article
- Striosomes control dopamine via dual pathways paralleling canonical basal ganglia circuits.Current biology : CB · 2024Article
- Current advance of nanotechnology in diagnosis and treatment for malignant tumors.Signal transduction and targeted therapy · 2024Review
- Striosomes Target Nigral Dopamine-Containing Neurons via Direct-D1 and Indirect-D2 Pathways Paralleling Classic Direct-Indirect Basal Ganglia Systems.bioRxiv : the preprint server for biology · 2024Article
- New rabies viral resources for multi-scale neural circuit mapping.Molecular psychiatry · 2024Article
53 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
22 authors at 3 institutions in 1 country.
Funding
Abstract
Recombinant rabies viral vectors have proven useful for applications including retrograde targeting of projection neurons and monosynaptic tracing, but their cytotoxicity has limited their use to short-term experiments. Here we introduce a new class of double-deletion-mutant rabies viral vectors that left transduced cells alive and healthy indefinitely. Deletion of the viral polymerase gene abolished cytotoxicity and reduced transgene expression to trace levels but left vectors still able to retrogradely infect projection neurons and express recombinases, allowing downstream expression of other transgene products such as fluorophores and calcium indicators. The morphology of retrogradely targeted cells appeared unperturbed at 1 year postinjection. Whole-cell patch-clamp recordings showed no physiological abnormalities at 8 weeks. Longitudinal two-photon structural and functional imaging in vivo, tracking thousands of individual neurons for up to 4 months, showed that transduced neurons did not die but retained stable visual response properties even at the longest time points imaged.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.