ArticleNature structural & molecular biology2018
Ribothrypsis, a novel process of canonical mRNA decay, mediates ribosome-phased mRNA endonucleolysis.
Article in Nature structural & molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Characterization of METTL3/14-mediated m6A modification in human transcriptome using Nanopore direct RNA sequencing.PLoS genetics · 2026Article
- Stalled translation on transcripts cleaved by RNase L activates signaling important for innate immunity.RNA (New York, N.Y.) · 2026Article
- Human CCR4 deadenylase homolog Angel1 is a non-stop mRNA decay factor.RNA (New York, N.Y.) · 2025Article
- Stalled translation on transcripts cleaved by RNase L activates signaling important for innate immunity.bioRxiv : the preprint server for biology · 2025Article
- Defining the True Native Ends of RNAs at Single-Molecule Level with TERA-Seq.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Full-length direct RNA sequencing uncovers stress granule-dependent RNA decay upon cellular stress.eLife · 2024Article
- Full-length direct RNA sequencing uncovers stress-granule dependent RNA decay upon cellular stress.bioRxiv : the preprint server for biology · 2024Article
- Boric acid intercepts 80S ribosome migration from AUG-stop by stabilizing eRF1.Nature chemical biology · 2024Article
- Translation-dependent and -independent mRNA decay occur through mutually exclusive pathways defined by ribosome density during T cell activation.Genome research · 2024Article
- Article
- CD95/Fas ligand mRNA is toxic to cells through more than one mechanism.Molecular biomedicine · 2023Article
- CD95/Fas ligand induced toxicity.Biochemical Society transactions · 2023Article
- Single-molecule imaging reveals translation-dependent destabilization of mRNAs.Molecular cell · 2023Article
- The identification of conserved sequence features of co-translationally decayed mRNAs and upstream open reading frames in angiosperm transcriptomes.Plant direct · 2023Article
- Cytoplasmic regulation of chloroplast ROS accumulation during effector-triggered immunity.Frontiers in plant science · 2023Review
- The landscape of translational stall sites in bacteria revealed by monosome and disome profiling.RNA (New York, N.Y.) · 2022Article
- Innate, translation-dependent silencing of an invasive transposon in Arabidopsis.EMBO reports · 2022Article
- Transcriptomic complexity of the human malaria parasite Plasmodium falciparum revealed by long-read sequencing.PloS one · 2022Article
- TERA-Seq: true end-to-end sequencing of native RNA molecules for transcriptome characterization.Nucleic acids research · 2021Article
- Ribosome dynamics and mRNA turnover, a complex relationship under constant cellular scrutiny.Wiley interdisciplinary reviews. RNA · 2021Review
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Abstract
mRNAs transmit the genetic information that dictates protein production and are a nexus for numerous pathways that regulate gene expression. The prevailing view of canonical mRNA decay is that it is mediated by deadenylation and decapping followed by exonucleolysis from the 3' and 5' ends. By developing Akron-seq, a novel approach that captures the native 3' and 5' ends of capped and polyadenylated RNAs, respectively, we show that canonical human mRNAs are subject to repeated cotranslational and ribosome-phased endonucleolytic cuts at the exit site of the mRNA ribosome channel, in a process that we term ribothrypsis. We uncovered RNA G quadruplexes among likely ribothrypsis triggers and show that ribothrypsis is a conserved process. Strikingly, we found that mRNA fragments are abundant in living cells and thus have important implications for the interpretation of experiments, such as RNA-seq, that rely on the assumption that mRNAs exist largely as full-length molecules in vivo.
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