ArticleeLife2018
Neutrophil-generated HOCl leads to non-specific thiol oxidation in phagocytized bacteria.
Article in eLife, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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Who cites it
32 citing papers in PubMed, 67 citations in OpenAlex.
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- Spatiotemporal analysis of reactive oxygen species using specific activity-based sensing fluorescent imaging probes.Nature reviews. Chemistry · 2026Review
- Graphene Quantum Dots Mitigate Oxidative Stress in Bacteria.bioRxiv : the preprint server for biology · 2026Article
- Oxidative Stress in Health and Disease: Mechanisms and Therapeutic Perspectives.International journal of molecular sciences · 2026Review
- Two redox-responsive LysR-type transcription factors control the oxidative stress response of Agrobacterium tumefaciens.Nucleic acids research · 2025Article
- SydR, a redox-sensing MarR-type regulator ofmBio · 2024Article
- New Insights Regarding the Use of Relevant Synthetic Compounds in Dentistry.Molecules (Basel, Switzerland) · 2024Review
- The Intricate Balance between Life and Death: ROS, Cathepsins, and Their Interplay in Cell Death and Autophagy.International journal of molecular sciences · 2024Review
- trans-Endothelial neutrophil migration activates bactericidal function via Piezo1 mechanosensing.Immunity · 2024Article
- The role of glutathione in periplasmic redox homeostasis and oxidative protein folding in Escherichia coli.Redox biology · 2023Article
- Human blood neutrophils generate ROS through FcγR-signaling to mediate protection against febrile P. falciparum malaria.Communications biology · 2023Article
- The oxidative stress response, in particular the katY gene, is temperature-regulated in Yersinia pseudotuberculosis.PLoS genetics · 2023Article
- Article
- Hybrid Biomaterial Initiates Refractory Wound Healing via Inducing Transiently Heightened Inflammatory Responses.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Ecology and Function of the Transmissible Locus of Stress Tolerance in Escherichia coli and Plant-AssociatedmSystems · 2021Article
- A Bacterial Inflammation Sensor Regulates c-di-GMP Signaling, Adhesion, and Biofilm Formation.mBio · 2021Article
- Induction of the reactive chlorine-responsive transcription factor RclR in Escherichia coli following ingestion by neutrophils.Pathogens and disease · 2021Article
- The effects of neutrophil-generated hypochlorous acid and other hypohalous acids on host and pathogens.Cellular and molecular life sciences : CMLS · 2021Review
- In Vivo Imaging with Genetically Encoded Redox Biosensors.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phagocytic immune cells kill pathogens in the phagolysosomal compartment with a cocktail of antimicrobial agents. Chief among them are reactive species produced in the so-called oxidative burst. Here, we show that bacteria exposed to a neutrophil-like cell line experience a rapid and massive oxidation of cytosolic thiols. Using roGFP2-based fusion probes, we could show that this massive breakdown of the thiol redox homeostasis was dependent on phagocytosis, presence of NADPH oxidase and ultimately myeloperoxidase. Interestingly, the redox-mediated fluorescence change in bacteria expressing a glutathione-specific Grx1-roGFP2 fusion protein or an unfused roGFP2 showed highly similar reaction kinetics to the ones observed with roGFP2-Orp1, under all conditions tested. We recently observed such an indiscriminate oxidation of roGFP2-based fusion probes by HOCl with fast kinetics in vitro. In line with these observations, abating HOCl production in immune cells with a myeloperoxidase inhibitor significantly attenuated the oxidation of all three probes in bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.