ArticlePloS one2018
Purification and characterization of the first γ-phospholipase inhibitor (γPLI) from Bothrops jararaca snake serum.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Spatiotemporal assessment of pathogenicJournal of water and health · 2024Article
- Proteomics and life-history variability of Endogenous Phospholipases A2 Inhibitors (PLIs) in Bothrops jararaca plasma.PloS one · 2024Article
- Functional Characterization and Anti-Tumor Effect of a Novel Group II Secreted Phospholipase AMolecules (Basel, Switzerland) · 2023Article
- Purification, Characterization and Evaluation of the Antitumoral Activity of a Phospholipase A2 from the SnakePharmaceuticals (Basel, Switzerland) · 2022Article
- Development of aqueous two-phase systems-based approaches for the selective recovery of metalloproteases and phospholipases ABioresources and bioprocessing · 2021Article
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- Identification and characterization of the first endogenous phospholipase AThe journal of venomous animals and toxins including tropical diseases · 2020Article
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- Comparative compositional and functional analyses of Bothrops moojeni specimens reveal several individual variations.PloS one · 2019Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phospholipases A2 (PLA2) are enzymes acting on the cell membrane phospholipids resulting in fatty acids and lysophospholipids and deconstructing the cell membrane. This protein is commonly found in snake venoms, causing tissue inflammation in the affected area. Evidence indicates that snakes have natural resistance to their own venom due to protective properties in plasma, that inhibit the action of proteins present in their venom. Given that, this study aimed to purify and characterize a γPLI from Bothrops jararaca serum, named γBjPLI. PLA2 inhibitor was isolated using two chromatographic steps: an ion exchange column (DEAE), followed by an affinity column (crotoxin coupled to a CNBr-activated Sepharose resin). The purity and biochemical characterization of the isolated protein were analyzed by RP-HPLC, SEC, SDS-PAGE, circular dichroism and mass spectrometry. The ability to inhibit PLA2 was determined by enzymatic activity, neutralization of paw edema and myonecrosis. The protein purity was confirmed by RP-HPLC and SEC, whilst an apparent molecular mass of 25 kDa and 20 kDa was obtained by SDS-PAGE, under reducing and non-reducing conditions, respectively. According to mass spectrometry analysis, this protein showed 72% and 68% of coverage when aligned to amino acid sequences of two proteins already described as PLIs. Thus, the inhibitory activity of enzymatic, edema and myonecrotic activities by γBjPLI suggests a role of this inhibitor for protection of these snakes against self-envenomation.
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