ArticleCell host & microbe2018
Dicer-2-Dependent Generation of Viral DNA from Defective Genomes of RNA Viruses Modulates Antiviral Immunity in Insects.
Article in Cell host & microbe, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
83 citing papers in PubMed, 1 synthesis or guideline pooled it, 164 citations in OpenAlex.
- Transovarial transmission of mosquito-borne viruses: a systematic review.Frontiers in cellular and infection microbiology · 2023Pooled it
- Detection of Nora virus in infectedFly · 2026Article
- Vector competence acrossJournal of virology · 2026Review
- Concepts of RNA virus evolution for the design of better antiviral countermeasures.Nature reviews. Microbiology · 2026Review
- Locust cGAS-like Receptors Recognize Derivatives of a Gypsy Retrotransposon to Synergize with RNAi Against Viral Invasion.Insects · 2026Article
- Tolerance to persistent cell-fusing agent virus infection in Aedes aegypti mosquitoes correlates with maintenance of lipid reserves.BMC biology · 2026Article
- Circular nucleic acids at the host-virus interface: from immune modulation to therapeutic innovation.Frontiers in immunology · 2026Review
- Characterization of RNA interference in the cnidarian Nematostella vectensis reveals partial target silencing but lack of small RNA amplification.PLoS biology · 2026Article
- In vivo Dicer-2 interactome during viral infection reveals novel pro and antiviral factors in Drosophila melanogaster.PLoS pathogens · 2025Article
- Article
- Exploiting social traits for clinical applications in bacteria and viruses.npj antimicrobials and resistance · 2025Review
- Cell-Autonomous and Non-Cell-Autonomous Antiviral Immunity via siRNA-Directed RNAi inImmune discovery · 2025Article
- Article
- Chikungunya Virus RNA Secondary Structures Impact Defective Viral Genome Production.Microorganisms · 2024Article
- Double-stranded RNA induces antiviral transcriptional response through the Dicer-2/Ampk/FoxO axis in an arthropod.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Regulation of ubiquitination and antiviral activity of Cactin by deubiquitinase Usp14 inJournal of virology · 2024Article
- Review
- Mosquito transgenerational antiviral immunity is mediated by vertical transfer of virus DNA sequences and RNAi.iScience · 2024Article
- Open questions in the social lives of viruses.Journal of evolutionary biology · 2023Review
- Recognition of Arboviruses by the Mosquito Immune System.Biomolecules · 2023Review
23 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
18 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RNAi pathway confers antiviral immunity in insects. Virus-specific siRNA responses are amplified via the reverse transcription of viral RNA to viral DNA (vDNA). The nature, biogenesis, and regulation of vDNA are unclear. We find that vDNA produced during RNA virus infection of Drosophila and mosquitoes is present in both linear and circular forms. Circular vDNA (cvDNA) is sufficient to produce siRNAs that confer partially protective immunity when challenged with a cognate virus. cvDNAs bear homology to defective viral genomes (DVGs), and DVGs serve as templates for vDNA and cvDNA synthesis. Accordingly, DVGs promote the amplification of vDNA-mediated antiviral RNAi responses in infected Drosophila. Furthermore, vDNA synthesis is regulated by the DExD/H helicase domain of Dicer-2 in a mechanism distinct from its role in siRNA generation. We suggest that, analogous to mammalian RIG-I-like receptors, Dicer-2 functions like a pattern recognition receptor for DVGs to modulate antiviral immunity in insects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.