ArticleInternational journal of legal medicine2018
Does altered protein metabolism interfere with postmortem degradation analysis for PMI estimation?
Article in International journal of legal medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 2 of them syntheses that pooled it.
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Who cites it
11 citing papers in PubMed, 2 syntheses or guidelines pooled it, 22 citations in OpenAlex.
- Forensic applications of postmortem protein analysis in estimating the time since death: a systematic review.International journal of legal medicine · 2026Pooled it
- "Omics" and Postmortem Interval Estimation: A Systematic Review.International journal of molecular sciences · 2025Pooled it
- Postmortem interval estimation through skeletal muscle protein degradation: a comprehensive review.International journal of legal medicine · 2026Review
- Evaluation of Muscle Proteins for Estimating the Post-Mortem Interval in Veterinary Forensic Pathology.Animals : an open access journal from MDPI · 2023Article
- Combining with lab-on-chip technology and multi-organ fusion strategy to estimate post-mortem interval of rat.Frontiers in medicine · 2022Article
- Dismembered porcine limbs as a proxy for postmortem muscle protein degradation.International journal of legal medicine · 2021Article
- Caspase 9 and Caspase 3 Immunohistochemical Pattern in Skeletal and Cardiac Muscles at Different Times after Death: An Experimental Study on PMI Estimation.Diagnostics (Basel, Switzerland) · 2021Article
- PP2A-C may be a promising candidate for postmortem interval estimation.International journal of legal medicine · 2021Article
- Postmortem Protein Degradation as a Tool to Estimate the PMI: A Systematic Review.Diagnostics (Basel, Switzerland) · 2020Review
- Intra- and intermuscular variations of postmortem protein degradation for PMI estimation.International journal of legal medicine · 2020Article
- A field study to evaluate PMI estimation methods for advanced decomposition stages.International journal of legal medicine · 2020Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An accurate estimation of the postmortem interval (PMI) is a central aspect in forensic routine. Recently, a novel approach based on the analysis of postmortem muscle protein degradation has been proposed. However, a number of questions remain to be answered until sensible application of this method to a broad variety of forensic cases is possible. To evaluate whether altered in vivo protein metabolism interferes with postmortem degradation patterns, we conducted a comparative study. We developed a standardized animal degradation model in rats, and collected additional muscle samples from animals recovering from muscle injury and from rats with developed disuse muscle atrophy after induced spinal cord injury. All samples were analyzed by SDS-PAGE and Western blot, labeling well-characterized muscle proteins. Tropomyosin was found to be stable throughout the investigated PMI and no alterations were detected in regenerating and atrophic muscles. In contrast, significant predictable postmortem changes occurred in desmin and vinculin protein band patterns. While no significant deviations from native patterns were detected in at-death samples of disuse muscle atrophy, interestingly, samples of rats recovering from muscle injury revealed additional desmin and vinculin degradation bands that did not occur in this form in any of the examined postmortem samples regardless of PMI. It remains to be investigated whether in vivo-altered metabolism influences postmortem degradation kinetics or if such muscle samples undergo postmortem degradation in a regular fashion.
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