ArticleScientific reports2018
Structural and functional studies on Pseudomonas aeruginosa DspI: implications for its role in DSF biosynthesis.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Structural basis of the AlgU-MucAmBio · 2026Article
- Dual-functional quorum sensing signal synthases DspII and DspI coordinate virulence switch in Pseudomonas aeruginosa.Nature communications · 2026Article
- Molecular Mechanisms Underlying Medium-Chain Free Fatty Acid-Regulated Activity of the Phospholipase PlaF fromJACS Au · 2024Article
- Functional Amyloids in Pseudomonas aeruginosa Are Essential for the Proteome Modulation That Leads to Pathoadaptation in Pulmonary Niches.Microbiology spectrum · 2023Article
- Research on Diffusible Signal Factor-Mediated Quorum Sensing inMolecules (Basel, Switzerland) · 2023Review
- TheInternational journal of molecular sciences · 2023Article
- A Bacterial Isolate Capable of Quenching Both Diffusible Signal Factor- andJournal of agricultural and food chemistry · 2022Article
- Insights into Adaptive Mechanisms of Extreme Acidophiles Based on Quorum Sensing/Quenching-Related Proteins.mSystems · 2022Article
- Quorum sensing in human gut and food microbiomes: Significance and potential for therapeutic targeting.Frontiers in microbiology · 2022Review
- Genomic and Metabolic Characteristics of the Pathogenicity inInternational journal of molecular sciences · 2021Review
- Anti-Virulence Properties of Plant Species: Correlation between In Vitro Activity and Efficacy in a Murine Model of Bacterial Infection.Microorganisms · 2021Article
- The Small RNAs PA2952.1 and PrrH as Regulators of Virulence, Motility, and Iron Metabolism in Pseudomonas aeruginosa.Applied and environmental microbiology · 2021Article
- Article
- Tetradecanoic Acids With Anti-Virulence Properties Increase the Pathogenicity ofFrontiers in cellular and infection microbiology · 2020Article
- Structural basis of DSF recognition by its receptor RpfR and its regulatory interaction with the DSF synthase RpfF.PLoS biology · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
16 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DspI, a putative enoyl-coenzyme A (CoA) hydratase/isomerase, was proposed to be involved in the synthesis of cis-2-decenoic acid (CDA), a quorum sensing (QS) signal molecule in the pathogen Pseudomonas aeruginosa (P. aeruginosa). The present study provided a structural basis for the dehydration reaction mechanism of DspI during CDA synthesis. Structural analysis reveals that Glu126, Glu146, Cys127, Cys131 and Cys154 are important for its enzymatic function. Moreover, we show that the deletion of dspI results in a remarkable decreased in the pyoverdine production, flagella-dependent swarming motility, and biofilm dispersion as well as attenuated virulence in P. aeruginosa PA14. This study thus unravels the mechanism of DspI in diffusible signal factor (DSF) CDA biosynthesis, providing vital information for developing inhibitors that interfere with DSF associated pathogenicity in P. aeruginosa.
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