ArticleParasites & vectors2018
Bacterial microbiota of Aedes aegypti mosquito larvae is altered by intoxication with Bacillus thuringiensis israelensis.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Effects of mosquito control using the microbial agent Bacillus thuringiensis israelensis (Bti) on aquatic and terrestrial ecosystems: a systematic review.Environmental evidence · 2023Pooled it
- Bacterially expressed recombinant TMOF induces mortality and gut microbial alterations in Aedes albopictus larvae.Scientific reports · 2026Article
- A taste of one's own medicine:Frontiers in immunology · 2025Article
- Carry-over effects of Bacillus thuringiensis on tolerant Aedes albopictus mosquitoes.Parasites & vectors · 2024Article
- Revealing the microbiome diversity and biocontrol potential of field Aedes ssp.: Implications for disease vector management.PloS one · 2024Article
- Influence of microbiota-driven natural antibodies on dengue transmission.Frontiers in immunology · 2024Article
- A Culex quinquefasciatus strain resistant to the binary toxin from Lysinibacillus sphaericus displays altered enzyme activities and energy reserves.Parasites & vectors · 2023Article
- Effects of theFrontiers in microbiology · 2023Article
- Article
- Comparative Nanopore Sequencing-Based Evaluation of the Midgut Microbiota of the Summer Chafer (International journal of environmental research and public health · 2022Article
- Ingestion of spinosad-containing toxic sugar bait altersFrontiers in microbiology · 2022Article
- Host resistance to Bacillus thuringiensis is linked to altered bacterial community within a specialist insect herbivore.Molecular ecology · 2021Article
- Aedes aegypti continuously exposed to Bacillus thuringiensis svar. israelensis does not exhibit changes in life traits but displays increased susceptibility for Zika virus.Parasites & vectors · 2021Article
- Review
- Short-Term Selection to Diflubenzuron andInsects · 2021Article
- Nanopore-Sequencing Characterization of the Gut Microbiota ofPathogens (Basel, Switzerland) · 2021Article
- Tick Immune System: What Is Known, the Interconnections, the Gaps, and the Challenges.Frontiers in immunology · 2021Review
- Which Is Stronger? A Continuing Battle Between Cry Toxins and Insects.Frontiers in microbiology · 2021Review
- Natural Variation in Physicochemical Profiles and Bacterial Communities Associated with Aedes aegypti Breeding Sites and Larvae on Guadeloupe and French Guiana.Microbial ecology · 2021Article
- The Tripartite Interaction of Host Immunity-Toxins · 2020Review
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Authors and funding
10 authors.
Funding
Abstract
backgroundInsect microbiota is a dynamic microbial community that can actively participate in defense against pathogens. Bacillus thuringiensis (Bt) is a natural entomopathogen widely used as a bioinsecticide for pest control. Although Bt's mode of action has been extensively studied, whether the presence of microbiota is mandatory for Bt to effectively kill the insect is still under debate. An association between a higher tolerance and a modified microbiota was already evidenced but a critical point remained to be solved: is the modified microbiota a cause or a consequence of a higher tolerance to Bt?
methodsIn this study we focused on the mosquito species Aedes aegypti, as no work has been performed on Diptera on this topic to date, and on B. thuringiensis israelensis (Bti), which is used worldwide for mosquito control. To avoid using antibiotics to cure bacterial microbiota, mosquito larvae were exposed to an hourly increasing dose of Bti during 25 hours to separate the most susceptible larvae dying quickly from more tolerant individuals, with longer survival.
resultsDenaturing gradient gel electrophoresis (DGGE) fingerprinting revealed that mosquito larval bacterial microbiota was strongly affected by Bti infection after only a few hours of exposure. Bacterial microbiota from the most tolerant larvae showed the lowest diversity but the highest inter-individual differences. The proportion of Bti in the host tissue was reduced in the most tolerant larvae as compared to the most susceptible ones, suggesting an active control of Bti infection by the host.
conclusionsHere we show that a modified microbiota is associated with a higher tolerance of mosquitoes to Bti, but that it is rather a consequence of Bti infection than the cause of the higher tolerance. This study paves the way to future investigations aiming at unraveling the role of host immunity, inter-species bacterial competition and kinetics of host colonization by Bti that could be at the basis of the phenotype observed in this study.
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