ArticleMolecular and cellular probes2018
A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China.
Article in Molecular and cellular probes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Establishment and application of a visual nucleic acid detection method for parvovirus.BMC veterinary research · 2025Article
- Characterization, Quantification, and Molecular Identification of Co-Infection of Canine Parvovirus (CPV-2) Variants in Dogs Affected by Gastroenteritis in Ecuador During 2022-2023.Veterinary sciences · 2025Article
- Rapid detection of feline parvovirus using RAA-CRISPR/Cas12a-based lateral flow strip and fluorescence.Frontiers in microbiology · 2025Article
- Sex-differential non-specific effects of adjuvanted and non-adjuvanted rabies vaccines versus placebo on all-cause mortality in dogs (NERVE-Dog study): a study protocol for a randomized controlled trial with a nested case-control study.BMC veterinary research · 2022Article
- The Changes in Canine Parvovirus Variants over the Years.International journal of molecular sciences · 2022Article
- Simultaneous Detection and Differentiation of Clinically Relevant Relapsing FeverJournal of clinical microbiology · 2021Article
- First report of canine parvovirus molecular detection in Bangladesh.Veterinary world · 2021Article
- An Improved Polymerase Cross-Linking Spiral Reaction Assay for Rapid Diagnostic of Canine Parvovirus 2 Infection.Frontiers in veterinary science · 2020Article
- Development of a TaqMan Based Real-Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen.BioMed research international · 2020Article
- CRISPR-Cas13a mediated nanosystem for attomolar detection of canine parvovirus type 2.Chinese chemical letters = Zhongguo hua xue kuai bao · 2019Article
- Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis.BMC veterinary research · 2019Article
- Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2.BMC veterinary research · 2019Article
- Advances in Diagnostic Approaches for Viral Etiologies of Diarrhea: From the Lab to the Field.Frontiers in microbiology · 2019Review
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Authors and funding
11 authors.
Funding
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Abstract
Canine parvovirus (CPV) is an important pathogen in domestic dogs, and the original antigenic types CPV-2 and its variants, CPV-2a, 2b and 2c, are prevalent worldwide. A multiplex TaqMan real-time PCR method was developed for the detection and differentiation of four antigenic types of CPV. A set of primers and probes, CPV-305F/CPV-305R and CPV-2-305P (for CPV-2)/CPV-2a-305P (for CPV-2a, 2b and 2c), was able to differentiate CPV-2 and its variants (CPV-2a, 2b and 2c). Another set of primers and probes, CPV-426F/CPV-426R and CPV-2-426P (for CPV-2 and 2a)/CPV-2b-426P (for CPV-2b)/CPV-2c-426P (for CPV-2c), was able to differentiate CPV-2a (2), CPV-2b, and CPV-2c. With these primers and probes, the multiplex TaqMan real-time PCR assay detected effectively and differentiated CPV-2, 2a, 2b and 2c by two separate real-time PCRs. No cross reactivity was observed with canine distemper virus, canine adenovirus, and canine coronavirus. The detection limit of the assay is 10
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