ArticleMolecular oral microbiology2018
In vivo expression of proteases and protease inhibitor, a serpin, by periodontal pathogens at teeth and implants.
Article in Molecular oral microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
- Interspecies regulation of virulence through gingipain inhibition by a Tannerella forsythia serpin.EMBO reports · 2026Article
- Bacterial proteases and Bcl-2 proteins in peri-implant tissues: An exploratory cross-sectional study.Clinical oral investigations · 2026Article
- Peri-Implantitis and Periodontitis: Biological Convergence, Contextual Divergence.Journal of periodontal research · 2026Review
- Inflammatory Mediators in Peri-Implantitis: From Pathophysiology to Therapeutic Targets.Advances in experimental medicine and biology · 2026Review
- Comparative analysis of gingival crevicular fluid and peri-implant crevicular fluid by mid-infrared spectroscopy: a split mouth study.Clinical oral investigations · 2025Article
- A scoping review of diagnostic techniques used for the detection of peri-implantitis around endosseous dental implants.Frontiers in dental medicine · 2025Review
- Microbiological and molecular aspects of periodontitis pathogenesis: an infection-induced inflammatory condition.Frontiers in cellular and infection microbiology · 2025Review
- Oral Fluid Biomarkers for Peri-Implantitis: A Scoping Review.International dental journal · 2024Article
- Review
- Current Molecular, Cellular and Genetic Aspects of Peri-Implantitis Disease: A Narrative Review.Dentistry journal · 2023Review
- Cytokine expression in gingival crevicular fluid around teeth opposing dental implants and 3-unit fixed partial dentures in a cross-sectional study.Head & face medicine · 2023Article
- A unique network of attack, defence and competence on the outer membrane of the periodontitis pathogenChemical science · 2023Article
- Antimicrobial activity of Desplac® oral gel in the subgingival multispecies biofilm formation.Frontiers in microbiology · 2023Article
- Novel regimens of phytopolyphenols with cisplatin or memantine and ZnSOJournal of dental sciences · 2022Article
- Host mRNA Analysis of Periodontal Disease Patients Positive forInternational journal of molecular sciences · 2022Review
- Gut Serpinome: Emerging Evidence in IBD.International journal of molecular sciences · 2021Review
- Article
- Plasmin inhibition by bacterial serpin: Implications in gum disease.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Article
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Authors and funding
6 authors at 3 institutions in 4 countries.
Funding
Abstract
Porphyromonas gingivalis and Tannerella forsythia secrete proteases, gingipains and KLIKK-proteases. In addition, T. forsythia produces a serpin (miropin) with broad inhibitory spectrum. The aim of this pilot study was to determine the level of expression of miropin and individual proteases in vivo in periodontal and peri-implant health and disease conditions. Biofilm and gingival crevicular fluid (GCF)/ peri-implant sulcular fluid (PISF) samples were taken from healthy tooth and implant sites (n = 10), gingivitis and mucositis sites (n = 12), and periodontitis and peri-implantitis sites (n = 10). Concentration of interleukin-8 (IL-8), IL-1β and IL-10 in GCF was determined by enzyme-linked immunosorbent assay. Loads of P. gingivalis and T. forsythia and the presence of proteases and miropin genes were assessed in biofilm by quantitative PCR, whereas gene expression was estimated by quantitative RT-PCR. The presence of P. gingivalis and T. forsythia, as well as the level of IL-8 and IL-1β, were associated with disease severity in the periodontal and peri-implant tissues. In biofilm samples harboring T. forsythia, genes encoding proteases were found to be present at 72.4% for karilysin and 100% for other KLIKK-protease genes and miropin. At the same time, detectable mRNA expression of individual genes ranged from 20.7% to 58.6% of samples (for forsylisin and miropsin-1, respectively). In comparison with the T. forsythia proteases, miropin and the gingipains were highly expressed. The level of expression of gingipains was associated with those of miropin and certain T. forsythia proteases around teeth but not implants. Cumulatively, KLIKK-proteases and especially miropin, might play a role in pathogenesis of both periodontal and peri-implant diseases.
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