Evidence map›Paper›PMID 29497951›Full record

ArticleJournal of assisted reproduction and genetics2018

Expression of inhibitor proteins that control primordial follicle reserve decreases in cryopreserved ovaries after autotransplantation.

Soner Celik, Ferda Topal Celikkan, Sinan Ozkavukcu, Alp Can, Ciler Celik-Ozenci

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Article in Journal of assisted reproduction and genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Soner CelikDepartment of Histology and Embryology, School of Medicine, Akdeniz University, Campus, 07070, Antalya, Turkey.
Ferda Topal CelikkanDepartment of Histology and Embryology, Ankara University School of Medicine, 06100, Ankara, Turkey.
Sinan OzkavukcuDepartment of Obstetrics and Gynecology, Centre for Assisted Reproduction, School of Medicine, Ankara University, 06100, Ankara, Turkey.
Alp CanDepartment of Histology and Embryology, Ankara University School of Medicine, 06100, Ankara, Turkey.
Ciler Celik-OzenciDepartment of Histology and Embryology, School of Medicine, Akdeniz University, Campus, 07070, Antalya, Turkey. cilerozenci@akdeniz.edu.tr.
Ankara University · TRAkdeniz University · TR

Funding

Akdeniz University Scientific Research Projects 2013.02.0122.013
6 · The paper itself

Abstract

purposeEven with 86 live births reported globally so far, the mechanism of primordial follicle loss following autotransplantation of the frozen-thawed ovarian tissue needs further evaluation. Pten, Tsc1, p27, and Amh are the inhibitor proteins that play crucial roles in suppressing the transition from the primordial follicle to primary state, maintaining the primordial follicle reserve. In this study, we aimed to evaluate whether the expression patterns of these proteins change and it may be related to the global primordial follicle loss after autotransplantation of the frozen-thawed ovarian tissue.

methodsFour groups were established in rats: fresh-control, frozen/thawed, fresh-transplanted, and frozen/thawed and transplanted. After slow freezing and thawing process, two ovarian pieces were transplanted into the back muscle of the same rat. After 2 weeks, grafts were harvested, fixed, and embedded into the paraffin block. Normal and atretic primordial/growing follicle count was performed in all groups. Ovarian tissues were evaluated for the dynamic expressions of the Pten, Tsc1, p27, and Amh proteins using immunohistochemistry, and H-score analyses were done.

resultsOvarian tissue cryopreservation does not change the expression patterns of inhibitory proteins that control ovarian reserve. Both in fresh and frozen/thawed autotransplanted groups, the expression of inhibitory proteins and Amh decreased significantly in primordial follicles and in growing follicles, respectively. In control group and in frozen/thawed group, primordial follicle counts were similar but decreased by almost half in both fresh-transplanted and frozen/thawed and transplanted groups.

conclusionsOne of the causes of primordial follicle loss after transplantation of ovarian graft may be decreased expression of the inhibitory proteins that guard the ovarian reserve and transplantation itself seems to be the major cause for disruption of inhibitory molecular signaling. Our findings highlight important molecular aspects for future clinical applications for fertility preservation in humans.

Indexed as

AnimalsCryopreservationCyclin-Dependent Kinase Inhibitor p27FemaleFertility PreservationOvarian FollicleOvarian ReservePTEN PhosphohydrolaseRatsRats, WistarReceptors, PeptideReceptors, Transforming Growth Factor betaTransplantation, AutologousTuberous Sclerosis Complex 1 ProteinTumor Suppressor Proteinsanti-Mullerian hormone receptorCdkn1b protein, ratCyclin-Dependent Kinase Inhibitor p27PTEN PhosphohydrolasePten protein, ratReceptors, PeptideReceptors, Transforming Growth Factor betaTSC1 protein, humanTsc1 protein, ratTuberous Sclerosis Complex 1 ProteinTumor Suppressor ProteinsAMHOvarian tissue cryopreservationp27Primordial folliclePtenTsc1

Identifiers

PMID29497951
PMCPMC5949118
OpenAlexW2789512964

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