ArticleFrontiers in cellular and infection microbiology2018
High-Throughput Quantification of Bacterial-Cell Interactions Using Virtual Colony Counts.
Article in Frontiers in cellular and infection microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Stochastic colonization and host-to-host transmission shape gut bacterial variability.bioRxiv : the preprint server for biology · 2026Article
- Evaluation of antimicrobial and antibiofilm activities of peptide Impatiens balsamina-M1 and Zinc oxide nanoparticles against Helicobacter pylori.Scientific reports · 2025Article
- The Antimicrobial Efficacy of Sodium Hypochlorite and Chlorhexidine in Gutta-Percha Cone Decontamination: A Systematic Review.Materials (Basel, Switzerland) · 2025Review
- Subversion of a family of antimicrobial proteins byFrontiers in cellular and infection microbiology · 2024Article
- Synthesis, Characterization and Potential Antimicrobial Activity of Selenium Nanoparticles Stabilized with Cetyltrimethylammonium Chloride.Nanomaterials (Basel, Switzerland) · 2023Article
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- Illuminating the oral microbiome: cellular microbiology.FEMS microbiology reviews · 2023Article
- Targeted dose delivery of Mycobacterium tuberculosis in mice using silicon antifoaming agent via aerosol exposure system.PloS one · 2022Article
- Serotype-dependent adhesion of Shiga toxin-producingFrontiers in microbiology · 2022Article
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- A simple, fast and reliable scan-based technique as a novel approach to quantify intracellular bacteria.BMC microbiology · 2019Article
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The quantification of bacteria in cell culture infection models is of paramount importance for the characterization of host-pathogen interactions and pathogenicity factors involved. The standard to enumerate bacteria in these assays is plating of a dilution series on solid agar and counting of the resulting colony forming units (CFU). In contrast, the virtual colony count (VCC) method is a high-throughput compatible alternative with minimized manual input. Based on the recording of quantitative growth kinetics, VCC relates the time to reach a given absorbance threshold to the initial cell count using a series of calibration curves. Here, we adapted the VCC method using the model organism
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