ArticleScientific reports2018
Fatty Acid Supplementation Reverses the Small Colony Variant Phenotype in Triclosan-Adapted Staphylococcus aureus: Genetic, Proteomic and Phenotypic Analyses.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 41 citations in OpenAlex.
- The cellular esterase FrmB controls metabolic homeostasis and small colony variant formation inbioRxiv : the preprint server for biology · 2026Article
- Comprehensive evaluation of the bactericidal and anti-pathogenic efficacy of chemical disinfectants against antibiotic resistant ESKAPE clinical strains.BMC microbiology · 2025Article
- Detection, Identification and Diagnostic Characterization of the Staphylococcal Small Colony-Variant (SCV) Phenotype.Antibiotics (Basel, Switzerland) · 2023Review
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- Subpopulations in Strains ofAntibiotics (Basel, Switzerland) · 2023Review
- Effects of Repeated in-vitro Exposure to Saudi Honey on Bacterial Resistance to Antibiotics and Biofilm Formation.Infection and drug resistance · 2023Article
- Effect of endocrine disruptors on bacterial virulence.Frontiers in cellular and infection microbiology · 2023Review
- Triclosan-resistant small-colony variants ofMicrobiology (Reading, England) · 2023Article
- Antimicrobial Potential ofBioMed research international · 2023Article
- High frequency of increased triclosan MIC among CC5 MRSA and risk of misclassification of the SCCmec into types.The Journal of antimicrobial chemotherapy · 2022Article
- FunctionalJournal of bacteriology · 2022Article
- The Antimicrobial Effects of Saudi Sumra Honey against Drug Resistant Pathogens: Phytochemical Analysis, Antibiofilm, Anti-Quorum Sensing, and Antioxidant Activities.Pharmaceuticals (Basel, Switzerland) · 2022Article
- Characteristics of Staphylococcus aureus small colony variants isolated from wound specimen of a tertiary care hospital in China.Journal of clinical laboratory analysis · 2022Article
- A functional menadione biosynthesis pathway is required for capsule production byMicrobiology (Reading, England) · 2021Article
- Extracellular DNA released by glycine-auxotrophic Staphylococcus epidermidis small colony variant facilitates catheter-related infections.Communications biology · 2021Article
- Galleria mellonella larvae exhibit a weight-dependent lethal median dose when infected with methicillin-resistant Staphylococcus aureus.Pathogens and disease · 2021Article
- Review
- Nonactive-Site Mutations inACS omega · 2020Article
- Persistence ofFrontiers in microbiology · 2020Review
- Exposure to a Manuka Honey Wound Gel Is Associated With Changes in Bacterial Virulence and Antimicrobial Susceptibility.Frontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Staphylococcus aureus can develop a small colony variant (SCV) phenotype in response to sub-lethal exposure to the biocide triclosan. In the current study, whole genome sequencing was performed and changes in virulence were investigated in five Staphylococcus aureus strains following repeated exposure to triclosan. Following exposure, 4/5 formed SCV and exhibited point mutations in the triclosan target gene fabI with 2/4 SCVs showing mutations in both fabI and fabD. The SCV phenotype was in all cases immediately reversed by nutritional supplementation with fatty acids or by repeated growth in the absence of triclosan, although fabI mutations persisted in 3/4 reverted SCVs. Virulence, determined using keratinocyte invasion and Galleria mellonella pathogenicity assays was significantly (p < 0.05) attenuated in 3/4 SCVs and in the non-SCV triclosan-adapted bacterium. Proteomic analysis revealed elevated FabI in 2/3 SCV and down-regulation in a protein associated with virulence in 1/3 SCV. In summary, attenuated keratinocyte invasion and larval virulence in triclosan-induced SCVs was associated with decreases in growth rate and virulence factor expression. Mutation occurred in fabI, which encodes the main triclosan target in all SCVs and the phenotype was reversed by fatty acid supplementation, demonstrating an association between fatty acid metabolism and triclosan-induced SCV.
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