Evidence map›Paper›PMID 29497017›Full record

ArticleActa crystallographica. Section F, Structural biology communications2018

Cloning, purification and structure determination of the HIV integrase-binding domain of lens epithelium-derived growth factor.

Clare Hannon, Abimael Cruz-Migoni, Olga Platonova, Robin L Owen, Joanne E Nettleship, Ami Miller, Stephen B Carr, Gemma Harris, Terence H Rabbitts, Simon E V Phillips

Abstract read
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In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Clare HannonWeatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, England.
Abimael Cruz-MigoniWeatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, England.
Olga PlatonovaWeatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, England.ORCID 0000-0002-0541-9578
Robin L OwenDiamond Light Source, Rutherford Appleton Laboratory, Didcot OX11 0DE, England.ORCID 0000-0002-2104-7057
Joanne E NettleshipOxford Protein Production Facility, Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot OX11 0FA, England.ORCID 0000-0002-1258-1733
Ami MillerWeatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, England.
Stephen B CarrDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England.
Gemma HarrisResearch Complex at Harwell, Rutherford Appleton Laboratory, Didcot OX11 0FA, England.
Terence H RabbittsWeatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, England.
Simon E V PhillipsDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England.

Funding

Bloodwise 12051Candlelighters 2008-2011Medical Research Council MR/J000612/1Medical Research Council MR/K018779/1Wellcome TrustWellcome Trust 099246/Z/12/Z
6 · The paper itself

Abstract

Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding partner of HIV-1 integrase in human cells. The crystal structure of the HIV integrase-binding domain (IBD) of LEDGF has been determined in the absence of ligand. IBD was overexpressed in Escherichia coli, purified and crystallized by sitting-drop vapour diffusion. X-ray diffraction data were collected at Diamond Light Source to a resolution of 2.05 Å. The crystals belonged to space group P2

Indexed as

Adaptor Proteins, Signal TransducingAmino Acid SequenceCatalytic DomainCrystallizationCrystallography, X-RayHIV IntegraseHumansModels, MolecularProtein ConformationTranscription FactorsAdaptor Proteins, Signal TransducingHIV IntegrasePSIP1 protein, humanTranscription Factorsdomain swappingHIV integrase-binding domainhuman immunodeficiency viruslens epithelium-derived growth factor

Identifiers

PMID29497017
PMCPMC5947699

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.