ArticleThe Journal of cell biology2018
An ordered pattern of Ana2 phosphorylation by Plk4 is required for centriole assembly.
Article in The Journal of cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- CDK1 and CEP97 cooperatively control centriole length to orchestrate ciliogenesis and developmental patterning.Genes & development · 2026Article
- PLK4: Master Regulator of Centriole Duplication and Its Therapeutic Potential.Cytoskeleton (Hoboken, N.J.) · 2025Review
- The kinase ZYG-1 phosphorylates the cartwheel protein SAS-5 to drive centriole assembly in C. elegans.EMBO reports · 2024Article
- Generating CRISPR-edited clonal lines of culturedBiology methods & protocols · 2024Article
- A simple Turing reaction-diffusion model explains how PLK4 breaks symmetry during centriole duplication and assembly.PLoS biology · 2023Article
- Cep104 is a component of the centriole distal tip complex that regulates centriole growth and contributes to Drosophila spermiogenesis.Current biology : CB · 2023Article
- Polo-like kinase 4 homodimerization and condensate formation regulate its own protein levels but are not required for centriole assembly.Molecular biology of the cell · 2023Article
- Analysis of a rare progeria variant of Barrier-to-autointegration factor in Drosophila connects centromere function to tissue homeostasis.Cellular and molecular life sciences : CMLS · 2023Article
- Plk4 Is a Novel Substrate of Protein Phosphatase 5.International journal of molecular sciences · 2023Article
- Centriole growth is limited by the Cdk/Cyclin-dependent phosphorylation of Ana2/STIL.The Journal of cell biology · 2022Article
- Review
- The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis.Molecular biology of the cell · 2021Article
- Plk4 triggers autonomous de novo centriole biogenesis and maturation.The Journal of cell biology · 2021Article
- The dimeric Golgi protein Gorab binds to Sas6 as a monomer to mediate centriole duplication.eLife · 2021Article
- Interaction interface in the C-terminal parts of centriole proteins Sas6 and Ana2.Open biology · 2020Article
- Article
- With Age Comes Maturity: Biochemical and Structural Transformation of a Human Centriole in the Making.Cells · 2020Review
- A molecular mechanism for the procentriole recruitment of Ana2.The Journal of cell biology · 2020Article
- The Singularity of the Drosophila Male Germ Cell Centriole: The Asymmetric Distribution of Sas4 and Sas6.Cells · 2020Article
- Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Polo-like kinase 4 (Plk4) initiates an early step in centriole assembly by phosphorylating Ana2/STIL, a structural component of the procentriole. Here, we show that Plk4 binding to the central coiled-coil (CC) of Ana2 is a conserved event involving Polo-box 3 and a previously unidentified putative CC located adjacent to the kinase domain. Ana2 is then phosphorylated along its length. Previous studies showed that Plk4 phosphorylates the C-terminal STil/ANa2 (STAN) domain of Ana2/STIL, triggering binding and recruitment of the cartwheel protein Sas6 to the procentriole assembly site. However, the physiological relevance of N-terminal phosphorylation was unknown. We found that Plk4 first phosphorylates the extreme N terminus of Ana2, which is critical for subsequent STAN domain modification. Phosphorylation of the central region then breaks the Plk4-Ana2 interaction. This phosphorylation pattern is important for centriole assembly and integrity because replacement of endogenous Ana2 with phospho-Ana2 mutants disrupts distinct steps in Ana2 function and inhibits centriole duplication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.