Evidence map›Paper›PMID 29496450›Full record

ArticleBiochemical and biophysical research communications2018

A unified mechanism for plant polyketide biosynthesis derived from in silico modeling.

Eamonn F Healy, Luis Cervantes, Barret Nabona, Jacob Williams

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Eamonn F HealyDepartment of Chemistry, St. Edward's University, Austin, TX 78704, USA. Electronic address: healy@stedwards.edu.
Luis CervantesDepartment of Chemistry, St. Edward's University, Austin, TX 78704, USA.
Barret NabonaDepartment of Chemistry, St. Edward's University, Austin, TX 78704, USA.
Jacob WilliamsDepartment of Chemistry, St. Edward's University, Austin, TX 78704, USA.
St. Edward's University · US

Funding

IRACDA Postdoctoral Program: Collaborative Opportunities for Research EducatorsK12GM102745 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI BRODBELT, JENNIFER S. · 2013 to 2017
$2.2M
NIGMS NIH HHS K12 GM102745
6 · The paper itself

Abstract

The polyketide synthases found in a variety of plants and fungi provide a varied source of biologically active compounds of pharmacological and medicinal interest. Stilbene synthase and chalcone synthase catalyze the formation of a common tetraketide intermediate, but use different cyclization mechanisms to produce distinct and separate natural products. While key structural differences have been identified to explain this functional diversity, a fuller explication of the factors responsible for this mechanistic disparity is required. Based on the energetics of our models of the bound tetraketides, and our structural analysis of the active sites we propose that a key tautomeric conversion provides a mechanistic framework common to both cyclizations. A previously unidentified active water molecule facilitates cyclization in chalcone synthase through a Claisen mechanism. Such a "Claisen switch" is comparable to the previously characterized "aldol switch" mechanism proposed for the biosynthesis of resveratrol in stilbene synthase.

Indexed as

AcyltransferasesCatalytic DomainComputer SimulationCyclizationModels, ChemicalPlantsPolyketidesPolyketide SynthasesWaterAcyltransferasesflavanone synthetasePolyketidesPolyketide SynthasesWaterAldol condensationChalcone synthaseClaisen condensationPolyketide biosynthesisResveratrolStilbene synthase

Identifiers

PMID29496450
PMCPMC6051414
OpenAlexW2791621162

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.