ReviewMicroorganisms2018
Inteins: Localized Distribution, Gene Regulation, and Protein Engineering for Biological Applications.
Review in Microorganisms, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Intein splicing efficiency and RadA levels can control the mode of archaeal DNA replication.Science advances · 2024Article
- Determinants of maturation of theJournal of bacteriology · 2024Article
- Massive intein content inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Efficient splicing of the CPE intein derived from directed evolution of theActa biochimica et biophysica Sinica · 2023Article
- Standard Intein Gene Expression Ramps (SIGER) for Protein-Independent Expression Control.ACS synthetic biology · 2023Article
- Calcimycin InhibitsACS infectious diseases · 2022Article
- A unique self-truncation of bacterial GH5 endoglucanases leads to enhanced activity and thermostability.BMC biology · 2022Article
- Drug Conjugation via Maleimide-Thiol Chemistry Does Not Affect Targeting Properties of Cysteine-Containing Anti-FGFR1 Peptibodies.Molecular pharmaceutics · 2022Article
- Intein-Mediated Protein Engineering for Cell-Based Biosensors.Biosensors · 2022Review
- SufB intein splicing in Mycobacterium tuberculosis is influenced by two remote conserved N-extein histidines.Bioscience reports · 2022Article
- The Evolution of Intein-Based Affinity Methods as Reflected in 30 years of Patent History.Frontiers in molecular biosciences · 2022Review
- The Effects of Self-cleavage Intein-ELK16 Tag in the Transcript Steric Hindrance of IFN.Indian journal of clinical biochemistry : IJCB · 2021Article
- Inteins in Science: Evolution to Application.Microorganisms · 2020Review
- Article
- Evolving a Peptide: Library Platforms and Diversification Strategies.International journal of molecular sciences · 2019Review
- Biotechnological Applications of Protein Splicing.Current protein & peptide science · 2019Review
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inteins are self-splicing polypeptides with an ability to excise themselves from flanking host protein regions with remarkable precision; in the process, they ligate flanked host protein fragments. Inteins are distributed sporadically across all three domains of life (bacteria, archaea, and unicellular eukaryotes). However, their apparent localized distribution in DNA replication, repair, and recombination proteins (the 3Rs), particularly in bacteria and archaea, is enigmatic. Our understanding of the localized distribution of inteins in the 3Rs, and their possible regulatory role in such distribution, is still only partial. Nevertheless, understanding the chemistry of post-translational self-splicing of inteins has opened up opportunities for protein chemists to modify, manipulate, and bioengineer proteins. Protein-splicing technology is adapted to a wide range of applications, starting with untagged protein purification, site-specific protein labeling, protein biotinylation, isotope incorporation, peptide cyclization, as an antimicrobial target, and so on. This review is focused on the chemistry of splicing; the localized distribution of inteins, particularly in the 3Rs and their possible role in regulating host protein function; and finally, the use of protein-splicing technology in various protein engineering applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.