Evidence map›Paper›PMID 29495429›Full record

ArticleNanomaterials (Basel, Switzerland)2018

Enhanced Delivery of Therapeutic siRNA into Glioblastoma Cells Using Dendrimer-Entrapped Gold Nanoparticles Conjugated with β-Cyclodextrin.

Jieru Qiu, Lingdan Kong, Xueyan Cao, Aijun Li, Ping Wei, Lu Wang, Serge Mignani, Anne-Marie Caminade, Jean-Pierre Majoral, Xiangyang Shi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nanomaterials (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed, 1 pooled it
4.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 1 synthesis or guideline pooled it, 93 citations in OpenAlex.

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  18. Small Interfering RNA for Gliomas Treatment: Overcoming Hurdles in Delivery.Frontiers in cell and developmental biology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 3 countries.

Jieru QiuDepartment of Radiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China. jieruq@outlook.com.
Lingdan KongCollege of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China. lingdankong@hotmail.com.
Xueyan CaoCollege of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China. caoxy_116@dhu.edu.cn.
Aijun LiCollege of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China. andrewaijun.lee@gmail.com.
Ping WeiCollege of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China. weiping876@hotmail.com.
Lu WangCollege of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China. wanglu2013@dhu.edu.cn.
Serge MignaniUniversité Paris Descartes, PRES Sorbonne Paris Cité, CNRS UMR 860, Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologique, 45, rue des Saints Peres, 75006 Paris, France. serge_mignani@orange.fr.
Anne-Marie CaminadeLaboratoire de Chimie de Coordination du CNRS, 205 Route de Narbonne, BP 44099, 31077 Toulouse CEDEX 4, France. anne-marie.caminade@lcc-toulouse.fr.
Jean-Pierre MajoralLaboratoire de Chimie de Coordination du CNRS, 205 Route de Narbonne, BP 44099, 31077 Toulouse CEDEX 4, France. majoral@lcc-toulouse.fr.
Xiangyang ShiDepartment of Radiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China. xshi@dhu.edu.cn.ORCID 0000-0001-6785-6645
Donghua University · CNCentre National de la Recherche Scientifique · FRTongji University · CNDélégation Paris 5 · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe a safe and highly effective non-viral vector system based on β-cyclodextrin (β-CD)-modified dendrimer-entrapped gold nanoparticles (Au DENPs) for improved delivery small interfering RNA (siRNA) to glioblastoma cells. In our approach, we utilized amine-terminated generation 5 poly(amidoamine) dendrimers partially grafted with β-CD as a nanoreactor to entrap Au NPs. The acquired β-CD-modified Au DENPs (Au DENPs-β-CD) were complexed with two different types of therapeutic siRNA (B-cell lymphoma/leukemia-2 (Bcl-2) siRNA and vascular endothelial growth factor (VEGF) siRNA). The siRNA compression ability of the Au DENPs-β-CD was evaluated by various methods. The cytocompatibility of the vector/siRNA polyplexes was assessed by viability assay of cells. The siRNA transfection capability of the formed Au DENPs-β-CD vector was evaluated by flow cytometric assay of the cellular uptake of the polyplexes and Western blot assays of the Bcl-2 and VEGF protein expression. Our data reveals that the formed Au DENPs-β-CD carrier enables efficiently delivery of siRNA to glioma cells, has good cytocompatibility once complexed with the siRNA, and enables enhanced gene silencing to inhibit the expression of Bcl-2 and VEGF proteins. The developed Au DENPs-β-CD vector may be used for efficient siRNA delivery to different biosystems for therapeutic purposes.

Indexed as

gene silencinggold nanoparticlesPAMAM dendrimerssiRNAβ-CD

Identifiers

PMID29495429
PMCPMC5869622
OpenAlexW2793428109

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.