ArticlePLoS pathogens2018
Wolbachia-mediated virus blocking in mosquito cells is dependent on XRN1-mediated viral RNA degradation and influenced by viral replication rate.
Article in PLoS pathogens, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 66 citations in OpenAlex.
- Wesselsbron virus at the livestock-human interface: A practical guide to disease recognition and diagnosis.One health (Amsterdam, Netherlands) · 2026Review
- Interaction of themBio · 2025Article
- Symbiotic Bacteria: Wolbachia, Midgut Microbiota in Mosquitoes and Their Importance for Vector Prevention Strategies.Microbial ecology · 2024Review
- Ticks and Tick-Borne Pathogens Circulating in Peri-Domestic Areas in Mainland Portugal.Microorganisms · 2024Article
- Review
- AntiviralmBio · 2024Article
- Evidence of Differences in Cellular Regulation ofViruses · 2024Article
- Sustained antiviral insulin signaling during West Nile virus infection results in viral mutations.Frontiers in cellular and infection microbiology · 2024Article
- The cellular lives of Wolbachia.Nature reviews. Microbiology · 2023Review
- Differences in proteome perturbations caused by the Wolbachia strain wAu suggest multiple mechanisms of Wolbachia-mediated antiviral activity.Scientific reports · 2023Article
- Review
- Article
- Differences in gene expression in field populations of Wolbachia-infected Aedes aegypti mosquitoes with varying release histories in northern Australia.PLoS neglected tropical diseases · 2023Article
- Article
- Developing Wolbachia-based disease interventions for an extreme environment.PLoS pathogens · 2023Article
- MicroRNAs reshape the immunity of insects in response to bacterial infection.Frontiers in immunology · 2023Review
- Analysis of Aedes aegypti microRNAs in response to Wolbachia wAlbB infection and their potential role in mosquito longevity.Scientific reports · 2022Article
- Transcriptome analysis of Aedes albopictus midguts infected by dengue virus identifies a gene network module highly associated with temperature.Parasites & vectors · 2022Article
- Transient Introgression ofPathogens (Basel, Switzerland) · 2022Article
- Differential viral RNA methylation contributes to pathogen blocking in Wolbachia-colonized arthropods.PLoS pathogens · 2022Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Wolbachia is currently being developed as a novel tool to block the transmission of dengue viruses (DENV) by Aedes aegypti. A number of mechanisms have been proposed to explain the DENV-blocking phenotype in mosquitoes, including competition for fatty acids like cholesterol, manipulation of host miRNAs and upregulation of innate immune pathways in the mosquito. We examined the various stages in the DENV infection process to better understand the mechanism of Wolbachia-mediated virus blocking (WMVB). Our results suggest that infection with Wolbachia does not inhibit DENV binding or cell entry, but reduces virus replication. In contrast to a previous report, we also observed a similar reduction in replication of West Nile virus (WNV). This reduced replication is associated with rapid viral RNA degradation in the cytoplasm. We didn't find a role for host miRNAs in WMVB. Further analysis showed that the 3' end of the virus subgenomic RNA was protected and accumulated over time suggesting that the degradation is XRN1-mediated. We also found that sub genomic flavivirus RNA accumulation inactivated XRN1 in mosquito cells in the absence of Wolbachia and led to enhancement of RNA degradation in its presence. Depletion of XRN1 decreased WMVB which was associated with a significant increase in DENV RNA. We also observed that WMVB is influenced by virus MOI and rate of virus replication. A comparatively elevated blocking was observed for slowly replicating DENV, compared to WNV. Similar results were obtained while analysing different DENV serotypes.
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