ArticlePloS one2018
The bovine TRPV3 as a pathway for the uptake of Na
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Menthol in Livestock: Unveiling Its Multifaceted Properties and Future Potential for Sustainable Agriculture.International journal of molecular sciences · 2025Review
- Prebiotics as modulators of colonic calcium and magnesium uptake.Acta physiologica (Oxford, England) · 2025Review
- Latest Insights into the In Vivo Studies in Murine Regarding the Role of TRP Channels in Wound Healing-A Review.International journal of molecular sciences · 2024Review
- Periparturient Mineral Metabolism: Implications to Health and Productivity.Animals : an open access journal from MDPI · 2024Review
- TRPV3 Ion Channel: From Gene to Pharmacology.International journal of molecular sciences · 2023Review
- Relationship between calcium dynamics and inflammatory status in the transition period of dairy cows.JDS communications · 2023Review
- Novel Insights into the Role of Keratinocytes-Expressed TRPV3 in the Skin.Biomolecules · 2023Review
- Effects of butyratePflugers Archiv : European journal of physiology · 2022Article
- Commentary on: Liebe, F., Liebe, H., Sponder, G., Mergler, S., Stumpff, F. "Effects of butyrate on ruminal CaPflugers Archiv : European journal of physiology · 2022Article
- Quantification of cytosol and membrane proteins in rumen epithelium of sheep with low or high CH4 emission phenotype.PloS one · 2022Article
- Beyond CaPflugers Archiv : European journal of physiology · 2021Article
- Structural mechanism of heat-induced opening of a temperature-sensitive TRP channel.Nature structural & molecular biology · 2021Article
- The TRPA1 Agonist Cinnamaldehyde Induces the Secretion of HCOInternational journal of molecular sciences · 2021Article
- Instant activation of TRP channels by NHCurrent research in physiology · 2020Article
- New ways for an old cation.Pflugers Archiv : European journal of physiology · 2020Article
- The TRPV3 channel of the bovine rumen: localization and functional characterization of a protein relevant for ruminal ammonia transport.Pflugers Archiv : European journal of physiology · 2020Article
- Dietary supplementation of menthol-rich bioactive lipid compounds alters circadian eating behaviour of sheep.BMC veterinary research · 2019Article
- Unravelling the secrets of the caecum.Pflugers Archiv : European journal of physiology · 2019Article
- Segmental differences in ion transport in rat cecum.Pflugers Archiv : European journal of physiology · 2019Article
- Dietary supplementation of essential oils in dairy cows: evidence for stimulatory effects on nutrient absorption.Animal : an international journal of animal bioscience · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Absorption of ammonia from the gastrointestinal tract results in problems that range from hepatic encephalopathy in humans to poor nitrogen efficiency of cattle with consequences for the global climate. Previous studies on epithelia and cells from the native ruminal epithelium suggest functional involvement of the bovine homologue of TRPV3 (bTRPV3) in ruminal NH4+ transport. Since the conductance of TRP channels to NH4+ has never been studied, bTRPV3 was overexpressed in HEK-293 cells and investigated using the patch-clamp technique and intracellular calcium imaging. Control cells contained the empty construct. Divalent cations blocked the conductance for monovalent cations in both cell types, with effects higher in cells expressing bTRPV3. In bTRPV3 cells, but not in controls, menthol, thymol, carvacrol, or 2-APB stimulated whole cell currents mediated by Na+, Cs+, NH4+, and K+, with a rise in intracellular Ca2+ observed in response to menthol. While only 25% of control patches showed single-channel events (with a conductance of 40.8 ± 11.9 pS for NH4+ and 25.0 ± 5.8 pS for Na+), 90% of bTRPV3 patches showed much larger conductances of 127.8 ± 4.2 pS for Na+, 240.1 ± 3.6 pS for NH4+, 34.0 ± 1.7 pS for Ca2+, and ~ 36 pS for NMDG+. Open probability, but not conductance, rose with time after patch excision. In conjunction with previous research, we suggest that bTRPV3 channels may play a role in the transport of Na+, K+, Ca2+ and NH4+ across the rumen with possible repercussions for understanding the function of TRPV3 in other epithelia.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.