ArticlePloS one2018
The effect of increasing the sulfation level of chondroitin sulfate on anticoagulant specific activity and activation of the kinin system.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Dynamics of Heparin Translocations Through Solid-State Nanopores.Electrophoresis · 2026Article
- Comprehensive anticoagulant effect of heparinoid in blood samples from patients with hemophilia.International journal of hematology · 2026Article
- Sulfated Polysaccharides in Wound Healing: From Bioactive Materials Design to Regenerative Therapeutics.International journal of nanomedicine · 2026Review
- Boosting chondrocyte bioactivity with ultra-sulfated glycopeptide supramolecular polymers.Acta biomaterialia · 2024Article
- Influence of Anticoagulants and Heparin Contaminants on the Suitability of MMP-9 as a Blood-Derived Biomarker.International journal of molecular sciences · 2024Article
- Efficient Expression and Characterization of an Endo-Type Lyase HCLase_M28 and Its Gradual Scale-Up Fermentation for the Preparation of Chondroitin Sulfate Oligosaccharides.Applied biochemistry and biotechnology · 2024Article
- Physicochemical Properties of Different Sulfated Polysaccharide Components fromJournal of fungi (Basel, Switzerland) · 2024Article
- Glycosaminoglycans fromMarine drugs · 2021Article
- Inhibition of BACE1, the β-secretase implicated in Alzheimer's disease, by a chondroitin sulfate extract fromNeural regeneration research · 2020Article
- Glycosaminoglycan-Inspired Biomaterials for the Development of Bioactive Hydrogel Networks.Molecules (Basel, Switzerland) · 2020Review
- Galactosaminoglycans: Medical Applications and Drawbacks.Molecules (Basel, Switzerland) · 2019Review
- By-Products of Heparin Production Provide a Diverse Source of Heparin-like and Heparan Sulfate Glycosaminoglycans.Scientific reports · 2019Article
- Heparins Sourced From Bovine and Porcine Mucosa Gain Exclusive Monographs in the Brazilian Pharmacopeia.Frontiers in medicine · 2019Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oversulfated chondroitin sulfate (OSCS) was identified as a contaminant in certain heparin preparations as the cause of adverse reactions in patients. OSCS was found to possess both plasma anticoagulant activity and the ability to activate prekallikrein to kallikrein. Differentially sulfated chondroitin sulfates were prepared by synthetic modification of chondroitin sulfate and were compared to the activity of OSCS purified from contaminated heparin. Whilst chondroitin sulfate was found to have minimal anticoagulant activity, increasing sulfation levels produced an anticoagulant response which we directly show for the first time is mediated through heparin cofactor II. However, the tetra-sulfated preparations did not possess any higher anticoagulant activity than several tri-sulfated variants, and also had lower heparin cofactor II mediated activity. Activation of prekallikrein was concentration dependent for all samples, and broadly increased with the degree of sulfation, though the di-sulfated preparation was able to form more kallikrein than some of the tri-sulfated preparations. The ability of the samples to activate the kinin system, as measured by bradykinin, was observed to be through kallikrein generation. These results show that whilst an increase in sulfation of chondroitin sulfate did cause an increase in anticoagulant activity and activation of the kinin system, there may be subtler structural interactions other than sulfation at play given the different responses observed.
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