ArticleJournal of molecular neuroscience : MN2018
Analysis of Housekeeping Genes for Accurate Normalization of qPCR Data During Early Postnatal Brain Development.
Article in Journal of molecular neuroscience : MN, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 13 citations in OpenAlex.
- A systematic review on the selection of reference genes for gene expression studies in rodents: are the classics the best choice?Molecular biology reports · 2024Pooled it
- Proteolytic Coherence and Trophic Divergence: Region-Specific Mapping of the tPA/PAI-1/uPAR and IGF/IGFBP-5 Responses to Kainic Acid-Induced Seizures.Neurochemical research · 2026Article
- Spatially resolved mapping of histones reveals selective neuronal response in Rett syndrome.The FEBS journal · 2026Article
- Article
- Evaluation of suitable reference genes for gene expression studies in the developing mouse cortex using RT-qPCR.BMC neuroscience · 2025Article
- The Vulnerability of the Developing Brain: Analysis of Highly Expressed Genes in Infant C57BL/6 Mouse Hippocampus in Relation to Phenotypic Annotation Derived From Mutational Studies.Bioinformatics and biology insights · 2022Article
- Article
- Reference Gene Validation in the Brain Regions of Young Rats after Pentylenetetrazole-Induced Seizures.Biomedicines · 2020Article
- Selection of Reliable Reference Genes for Analysis of Gene Expression in Spinal Cord during Rat Postnatal Development and after Injury.Brain sciences · 2019Article
- Identification of qPCR reference genes suitable for normalizing gene expression in the mdx mouse model of Duchenne muscular dystrophy.PloS one · 2019Article
- A brief procedure for big data analysis of gene expression.Animal models and experimental medicine · 2018Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Maturation of the neocortex during the first three postnatal weeks is a complex process that is characterized by different time courses of maturation of different areas of the neocortex. Analysis of gene expression using quantitative PCR after reverse transcription during this period of ontogeny and comparison of different cortical areas require optimal selection of reference genes for correct normalization of the data. Here, we compared expression of nine reference genes in the somatosensory and visual areas of the neocortex at the age of 5, 8, 10, 13, and 20 days. Using widely used GeNorm and NormFinder applications, as well as a novel approach, we compared stability of expression of GAPDH, YWHAZ, TFR1, RPS18, Rn18S, HPRT1, KIF5C, OSBP, and UQCRFS1. We found that, in both neocortical areas studied, YWHAZ and UQCRFS1 are the best reference genes whereas GAPDH and TFR1 are also stably expressed in the somatosensory cortex and OSBP is stable in the visual cortex. Additionally, analysis of stability of expression of these genes by a novel approach showed that the expression of these genes is stable during the entire period from the 5th to the 20th postnatal days.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.