ArticlePlanta2018
Gene editing by CRISPR/Cas9 in the obligatory outcrossing Medicago sativa.
Article in Planta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 66 citations in OpenAlex.
- CRISPR-Based genome editing in pulses: current approaches, challenges, and future prospects.Plant molecular biology · 2025Review
- Substantial enhancement ofHorticulture research · 2025Article
- Comprehensive benchmarking of genome editing quantification methods for plant applications.iScience · 2025Article
- Harnessing the Potential of CRISPR/Cas in Targeted Alfalfa Improvement for Stress Resilience.International journal of molecular sciences · 2025Review
- Latin America: a hub for agrobiotechnological innovations.Annals of botany · 2025Review
- Genome editing for grass improvement and future agriculture.Horticulture research · 2025Article
- CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in pigeonpea and groundnut.Functional & integrative genomics · 2024Article
- Functional Characterization of thePlants (Basel, Switzerland) · 2024Article
- Efficient multi-allelic genome editing via CRISPR-Cas9 ribonucleoprotein-based delivery toFrontiers in plant science · 2024Article
- Multiplex CRISPR/Cas9-mediated mutagenesis of alfalfa FLOWERING LOCUS Ta1 (MsFTa1) leads to delayed flowering time with improved forage biomass yield and quality.Plant biotechnology journal · 2023Article
- CRISPR/Cas9-mediated knockout of SPL13 radically increases lettuce yield.Plant cell reports · 2023Article
- Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences.Foods (Basel, Switzerland) · 2023Review
- Eliciting Targeted Mutations in Medicago sativa Using CRISPR/Cas9-Mediated Genome Editing: A Potential Tool for the Improvement of Disease Resistance.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate phosphate.G3 (Bethesda, Md.) · 2022Article
- Biofortification-A Frontier Novel Approach to Enrich Micronutrients in Field Crops to Encounter the Nutritional Security.Molecules (Basel, Switzerland) · 2022Review
- Targeted plant improvement through genome editing: from laboratory to field.Plant cell reports · 2021Review
- Expanding the Benefits ofFrontiers in plant science · 2021Article
- CRISPR/Cas9-Mediated Targeted Mutagenesis ofFrontiers in plant science · 2021Article
- The CRISPR/Cas9-Mediated Modulation ofFrontiers in plant science · 2021Article
- Digital PCR: What Relevance to Plant Studies?Biology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
MAIN
conclusionThe CRISPR/Cas9 technique was successfully used to edit the genome of the obligatory outcrossing plant species Medicago sativa L. (alfalfa). RNA-guided genome engineering using Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR)/Cas9 technology enables a variety of applications in plants. Successful application and validation of the CRISPR technique in a multiplex genome, such as that of M. sativa (alfalfa) will ultimately lead to major advances in the improvement of this crop. We used CRISPR/Cas9 technique to mutate squamosa promoter binding protein like 9 (SPL9) gene in alfalfa. Because of the complex features of the alfalfa genome, we first used droplet digital PCR (ddPCR) for high-throughput screening of large populations of CRISPR-modified plants. Based on the results of genome editing rates obtained from the ddPCR screening, plants with relatively high rates were subjected to further analysis by restriction enzyme digestion/PCR amplification analyses. PCR products encompassing the respective small guided RNA target locus were then sub-cloned and sequenced to verify genome editing. In summary, we successfully applied the CRISPR/Cas9 technique to edit the SPL9 gene in a multiplex genome, providing some insights into opportunities to apply this technology in future alfalfa breeding. The overall efficiency in the polyploid alfalfa genome was lower compared to other less-complex plant genomes. Further refinement of the CRISPR technology system will thus be required for more efficient genome editing in this plant.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.