ArticleBiotechnology for biofuels2018
Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance.
Article in Biotechnology for biofuels, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Lignin from Plant-Based Agro-Industrial Biowastes: From Extraction to Sustainable Applications.Polymers · 2025Review
- Article
- Automated quantification of fluorescence and morphological changes in pretreated wood cells by fluorescence macroscopy.Plant methods · 2023Article
- Apricot Seed Shells and Walnut Shells as Unconventional Sugars and Lignin Sources.Molecules (Basel, Switzerland) · 2023Article
- Production of butanol from lignocellulosic biomass: recent advances, challenges, and prospects.RSC advances · 2022Review
- Review
- Hydrothermal Pretreatment of Lignocellulosic Feedstocks to Facilitate Biochemical Conversion.Frontiers in bioengineering and biotechnology · 2022Review
- Evaluating polymer interplay after hot water pretreatment to investigate maize stem internode recalcitrance.Biotechnology for biofuels · 2021Article
- Bioethanol Production by Enzymatic Hydrolysis from Different Lignocellulosic Sources.Molecules (Basel, Switzerland) · 2021Review
- Low-Cost Cellulase-Hemicellulase Mixture Secreted byInternational journal of molecular sciences · 2020Article
- Novel Synergistic Mechanism for Lignocellulose Degradation by a Thermophilic Filamentous Fungus and a Thermophilic Actinobacterium Based on Functional Proteomics.Frontiers in microbiology · 2020Article
- Gene coexpression network analysis reveals a novel metabolic mechanism ofBiotechnology for biofuels · 2020Article
- Multiscale analysis of lignocellulose recalcitrance towards OrganoCat pretreatment and fractionation.Biotechnology for biofuels · 2020Article
- Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance.Biotechnology for biofuels · 2019Article
- Lignocellulosic Biomass: Understanding Recalcitrance and Predicting Hydrolysis.Frontiers in chemistry · 2019Review
- Omics-based analyses revealed metabolic responses ofBiotechnology for biofuels · 2019Article
- Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification.Biotechnology for biofuels · 2018Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundBiomass recalcitrance to enzymatic hydrolysis has been assigned to several structural and chemical factors. However, their relative importance remains challenging to evaluate. Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples. A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.
resultsThe pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification). Generic correlation coefficients were calculated between the measured chemical and structural features and the final saccharification rates. Increases in porosity displayed overall strong positive correlations with saccharification efficiency, but different porosity ranges were concerned depending on the considered biomass. Lignin-related factors displayed highly negative coefficients for all biomasses. Lignin content, which is likely involved in the correlations observed for porosity, was less detrimental to enzymatic hydrolysis than lignin composition. Lignin influence was highlighted by the strong negative correlation with fluorescence intensity which mainly originates from monolignols in mature tissues.
conclusionsOur results provide a better understanding of the factors responsible for biomass recalcitrance that can reasonably be considered as generic. The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin. Lignin composition, which probably influences its structure, is the most important parameter to overcome to enhance enzymes access to the polysaccharides. Accordingly, fluorescence intensity was found to be a rapid and simple method to assess recalcitrance after pretreatment.
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