ReviewFrontiers in microbiology2018
Protein Engineering for Nicotinamide Coenzyme Specificity in Oxidoreductases: Attempts and Challenges.
Review in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 70 citations in OpenAlex.
- A sequence motif enables widespread use of noncanonical redox cofactors in natural enzymes.Nature chemical biology · 2026Article
- Expanding the cofactor range of aldehyde reductase YqhD by mutating key amino acids.Biotechnology letters · 2026Article
- Kratom medium-chain dehydrogenase/reductase enzymes exhibit broad substrate and cofactor promiscuity.Journal of industrial microbiology & biotechnology · 2026Article
- Improving Cofactor Promiscuity of HMG-CoA Reductase fromBiomolecules · 2025Article
- Structural insights into the catalytic mechanism of the microcystin tailoring enzyme McyI.Communications biology · 2025Article
- Cofactor engineering for improved production of 2,4-dihydroxybutyric acid via the synthetic homoserine pathway.Frontiers in bioengineering and biotechnology · 2025Article
- Shifting redox reaction equilibria on demand using an orthogonal redox cofactor.Nature chemical biology · 2024Article
- A refined picture of the native amine dehydrogenase family revealed by extensive biodiversity screening.Nature communications · 2024Article
- Changing the Electron Acceptor Specificity ofInternational journal of molecular sciences · 2023Article
- A Hitchhiker's Guide to Supplying Enzymatic Reducing Power into Synthetic Cells.ACS synthetic biology · 2023Review
- NAD(P)H binding configurations revealed by time-resolved fluorescence and two-photon absorption.Biophysical journal · 2023Article
- Insertions and deletions mediated functional divergence of Rossmann fold enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Engineering Isopropanol Dehydrogenase for Efficient Regeneration of Nicotinamide Cofactors.Applied and environmental microbiology · 2022Article
- Protein cost minimization promotes the emergence of coenzyme redundancy.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Rossmann-toolbox: a deep learning-based protocol for the prediction and design of cofactor specificity in Rossmann fold proteins.Briefings in bioinformatics · 2022Article
- Growth-Based, High-Throughput Selection for NADH Preference in an Oxygen-Dependent Biocatalyst.ACS synthetic biology · 2021Article
- Article
- carba Nicotinamide Adenine Dinucleotide Phosphate: Robust Cofactor for Redox Biocatalysis.Angewandte Chemie (International ed. in English) · 2021Article
- An integrative approach to improving the biocatalytic reactions of whole cells expressing recombinant enzymes.World journal of microbiology & biotechnology · 2021Review
- Engineering the biomimetic cofactors of NMNH for cytochrome P450 BM3 based on binding conformation refinement.RSC advances · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxidoreductases are ubiquitous enzymes that catalyze an extensive range of chemical reactions with great specificity, efficiency, and selectivity. Most oxidoreductases are nicotinamide cofactor-dependent enzymes with a strong preference for NADP or NAD. Because these coenzymes differ in stability, bioavailability and costs, the enzyme preference for a specific coenzyme is an important issue for practical applications. Different approaches for the manipulation of coenzyme specificity have been reported, with different degrees of success. Here we present various attempts for the switching of nicotinamide coenzyme preference in oxidoreductases by protein engineering. This review covers 103 enzyme engineering studies from 82 articles and evaluates the accomplishments in terms of coenzyme specificity and catalytic efficiency compared to wild type enzymes of different classes. We analyzed different protein engineering strategies and related them with the degree of success in inverting the cofactor specificity. In general, catalytic activity is compromised when coenzyme specificity is reversed, however when switching from NAD to NADP, better results are obtained. In most of the cases, rational strategies were used, predominantly with loop exchange generating the best results. In general, the tendency of removing acidic residues and incorporating basic residues is the strategy of choice when trying to change specificity from NAD to NADP, and
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