ArticleNature communications2018
Long-term optical brain imaging in live adult fruit flies.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- A half-center oscillator regulates sleep pressure.Nature neuroscience · 2026Article
- Protocol for a saline-free surgical preparation of adult Drosophila for chronic in vivo brain imaging.STAR protocols · 2026Article
- Imaging Cell Competition in Ex-VivoMethods and protocols · 2025Article
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- A distinct neural ensemble to integrate contradictory information and form long-term memory inScience advances · 2025Article
- Dynamics of glia and neurons regulate homeostatic rest, sleep and feeding behavior in Drosophila.Nature neuroscience · 2025Article
- Article
- Wide-field fluorescence lifetime imaging of neuron spiking and subthreshold activity in vivo.Science (New York, N.Y.) · 2023Article
- Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types.Science (New York, N.Y.) · 2022Article
- Review
- Microengineered devices enable long-term imaging of the ventral nerve cord in behaving adult Drosophila.Nature communications · 2022Article
- Article
- Review
- A Model of Discovery: The Role of Imaging Established and Emerging Non-mammalian Models in Neuroscience.Frontiers in molecular neuroscience · 2022Review
- A pair of dopamine neurons mediate chronic stress signals to induce learning deficit inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Lighting Up CaCells · 2021Review
- Improving scalability in systems neuroscience.Neuron · 2021Review
- Navigation of a Freely Walking Fruit Fly in Infinite Space Using a Transparent Omnidirectional Locomotion Compensator (TOLC).Sensors (Basel, Switzerland) · 2021Article
- PhotoGal4: A Versatile Light-Dependent Switch for Spatiotemporal Control of Gene Expression in Drosophila Explants.iScience · 2020Article
- Investigating learning-related neural circuitry with chronic in vivo optical imaging.Brain structure & function · 2020Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Time-lapse in vivo microscopy studies of cellular morphology and physiology are crucial toward understanding brain function but have been infeasible in the fruit fly, a key model species. Here we use laser microsurgery to create a chronic fly preparation for repeated imaging of neural architecture and dynamics for up to 50 days. In fly mushroom body neurons, we track axonal boutons for 10 days and record odor-evoked calcium transients over 7 weeks. Further, by using voltage imaging to resolve individual action potentials, we monitor spiking plasticity in dopamine neurons of flies undergoing mechanical stress. After 24 h of stress, PPL1-α'3 but not PPL1-α'2α2 dopamine neurons have elevated spike rates. Overall, our chronic preparation is compatible with a broad range of optical techniques and enables longitudinal studies of many biological questions that could not be addressed before in live flies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.