ArticleScientific reports2018
Simulated microgravity inhibits cell focal adhesions leading to reduced melanoma cell proliferation and metastasis via FAK/RhoA-regulated mTORC1 and AMPK pathways.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
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Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it, 80 citations in OpenAlex.
- Effects of theInternational journal of molecular sciences · 2021Pooled it
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- Gravitational and mechanical forces shape mitochondrial translation.Nature communications · 2026Article
- Human Multipotent Mesenchymal Stromal Cells Exhibit Resistance to Long-Term Simulated Microgravity.Bulletin of experimental biology and medicine · 2026Article
- Microgravity and cancer cells: from molecular mechanisms to therapeutic strategies.Cell death discovery · 2026Review
- Stem cells in space: microgravity effects on stem cell fate and implications for regenerative medicine.NPJ microgravity · 2025Review
- Immune System-Tumor Crosstalk Under Microgravity: Mechanistic Insights, Challenges, and Translational Perspectives.Cancers · 2025Review
- Aging and Altered Gravity: A Cellular Perspective.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Review
- Microgravity alleviates low-dose radiation-induced non-targeted carcinogenic effects.NPJ microgravity · 2025Article
- Mechanometabolism: recent findings on the intersection of cell adhesion, cell migration, and metabolism.American journal of physiology. Cell physiology · 2025Review
- Application of Microgravity Experiments in Plastic Surgery: A Literature Review.Plastic and reconstructive surgery. Global open · 2025Article
- The mechanosensitive channel ELKIN1 regulates cellular adaptations to simulated microgravity.NPJ microgravity · 2025Article
- Focal Adhesion Kinase Alleviates Simulated Microgravity-Induced Inhibition of Osteoblast Differentiation by Activating Transcriptional Wnt/β-Catenin-BMP2-COL1 and Metabolic SIRT1-PGC-1α-CPT1A Pathways.International journal of molecular sciences · 2025Article
- The Formation of Stable Lung Tumor Spheroids during Random Positioning Involves Increased Estrogen Sensitivity.Biomolecules · 2024Article
- Microgravity as a Tool to Investigate Cancer Induction in Pleura Mesothelial Cells.Current issues in molecular biology · 2024Article
- Exploring the influence of microgravity on chemotherapeutic drug response in cancer: Unveiling new perspectives.Journal of cellular and molecular medicine · 2024Review
- Modeled microgravity unravels the roles of mechanical forces in renal progenitor cell physiology.Stem cell research & therapy · 2024Article
- Highly oriented hydrogels for tissue regeneration: design strategies, cellular mechanisms, and biomedical applications.Theranostics · 2024Review
- Microgravity triggers ferroptosis and accelerates senescence in the MG-63 cell model of osteoblastic cells.NPJ microgravity · 2023Article
- Review
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
Simulated microgravity (SMG) was reported to affect tumor cell proliferation and metastasis. However, the underlying mechanism is elusive. In this study, we demonstrate that clinostat-modelled SMG reduces BL6-10 melanoma cell proliferation, adhesion and invasiveness in vitro and decreases tumor lung metastasis in vivo. It down-regulates metastasis-related integrin α6β4, MMP9 and Met72 molecules. SMG significantly reduces formation of focal adhesions and activation of focal adhesion kinase (FAK) and Rho family proteins (RhoA, Rac1 and Cdc42) and of mTORC1 kinase, but activates AMPK and ULK1 kinases. We demonstrate that SMG inhibits NADH induction and glycolysis, but induces mitochondrial biogenesis. Interestingly, administration of a RhoA activator, the cytotoxic necrotizing factor-1 (CNF1) effectively converts SMG-triggered alterations and effects on mitochondria biogenesis or glycolysis. CNF1 also converts the SMG-altered cell proliferation and tumor metastasis. In contrast, mTORC inhibitor, rapamycin, produces opposite responses and mimics SMG-induced effects in cells at normal gravity. Taken together, our observations indicate that SMG inhibits focal adhesions, leading to inhibition of signaling FAK and RhoA, and the mTORC1 pathway, which results in activation of the AMPK pathway and reduced melanoma cell proliferation and metastasis. Overall, our findings shed a new light on effects of microgravity on cell biology and human health.
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