ArticleThe Journal of veterinary medical science2018
Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.
Article in The Journal of veterinary medical science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 7 citations in OpenAlex.
- A New Dual Fluorescence Method for Rapid Detection of Infectious Bronchitis Virus at Constant Temperature.Microorganisms · 2024Article
- The use of RT-PCR in the diagnosis and differentiation of vaccine strains of chicken infectious bronchitis and Newcastle disease.Open veterinary journal · 2023Article
- Pathological effect of infectious bronchitis disease virus on broiler chicken trachea and kidney tissues.Veterinary world · 2020Article
- An infection of Enterobacter ludwigii affects development and causes age-dependent neurodegeneration in Drosophila melanogaster.Invertebrate neuroscience : IN · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
A method based on Melting Temperature analysis of Hypervariable regions (HVR) of S1 gene within a RT-qPCR was developed to detect different genotypes of avian infectious bronchitis virus (IBV) and identify the Mass genotype. The method was able to rapidly identify the Mass genotype among IBV field isolates, vaccine attenuated strains and reference M41 strain in allantoic liquid and also directly in tissues. The RT-qPCR developed detected the virus in both tracheal and pulmonary samples from M41-infected or H120-infected birds, in a larger post-infection period compared to detection by standard method of virus isolation. RT-qPCR method tested provided a sensitivity and rapid approach for screening on IBV detection and Mass genotyping from IBV isolates.
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