Evidence map›Paper›PMID 29490563›Full record

ArticleTissue engineering. Part C, Methods2018

A Transgenic tdTomato Rat for Cell Migration and Tissue Engineering Applications.

Brian C Syverud, Jonathan P Gumucio, Brittany L Rodriguez, Olga M Wroblewski, Shelby E Florida, Christopher L Mendias, Lisa M Larkin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering. Part C, Methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Brian C Syverud1 Department of Biomedical Engineering, University of Michigan , Ann Arbor, Michigan.
Jonathan P Gumucio2 Department of Molecular and Integrative Physiology, University of Michigan , Ann Arbor, Michigan.
Brittany L Rodriguez1 Department of Biomedical Engineering, University of Michigan , Ann Arbor, Michigan.
Olga M Wroblewski1 Department of Biomedical Engineering, University of Michigan , Ann Arbor, Michigan.
Shelby E Florida2 Department of Molecular and Integrative Physiology, University of Michigan , Ann Arbor, Michigan.
Christopher L Mendias2 Department of Molecular and Integrative Physiology, University of Michigan , Ann Arbor, Michigan.
Lisa M Larkin1 Department of Biomedical Engineering, University of Michigan , Ann Arbor, Michigan.
University of Michigan–Ann Arbor · US

Funding

Tissue Engineering and RegenerationT32DE007057 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAVID H. KOHN · 1985 to 2026
$17.3M
Utilization of Engineered Skeletal Muscle Units to Repair Volumetric MuscleR01AR067744 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LARKIN, LISA M · 2015 to 2019
$2.3M
Fatty Macrophage Regulation of Atrophy and Inflammation in Rotator Cuff TearsF31AR065931 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI GUMUCIO, JONATHAN PAUL · 2014 to 2016
$103k
NIAMS NIH HHS F31 AR065931NIAMS NIH HHS R01 AR067744NIDCR NIH HHS T32 DE007057
6 · The paper itself

Abstract

The growing deficit in suitable tissues for patients awaiting organ transplants demonstrates the clinical need for engineered tissues as alternative graft sources. Demonstrating safety and efficacy by tracking the migration and fate of implanted cells is a key consideration required for approval of promising engineered tissues. Cells from transgenic animals that express green fluorescent protein (GFP) are commonly used for this purpose. However, GFP can create difficulties in practice due to high levels of green autofluorescence in many musculoskeletal tissues. Tandem-dimer tomato (tdTomato) is a stable, robust red fluorescent protein that is nearly threefold brighter than GFP. Our objective was to create a line of transgenic rats that ubiquitously express tdTomato in all cells, driven by the human ubiquitin C promoter. We sought to determine the rats' utility in tissue engineering applications by fabricating engineered skeletal muscle units (SMUs) from isolated muscle-derived tdTomato cells. These tdTomato SMUs were implanted into a volumetric muscle loss (VML) defect of the tibialis anterior muscle in a rat ubiquitously expressing GFP. We also evaluated a novel method for modularly combining individual SMUs to create a larger engineered tissue. Following a recovery period of 28 days, we found that implantation of the modular SMU led to a significant decrease in the size of the remaining VML deficit. Histological analysis of explanted tissues demonstrated both tdTomato and GFP expression in the repair site, indicating involvement of both implanted and host cells in the regeneration process. These results demonstrate the successful generation of a tdTomato transgenic rat, and the use of these rats in tissue engineering and cell migration applications. Furthermore, this study successfully validated a method for scaling engineered tissues to larger sizes, a factor that will be important for repairing volumetric injuries in more clinically relevant models.

Indexed as

Cell MovementTransgenesAnimalsCell SeparationCell TrackingFluorescenceGreen Fluorescent ProteinsImplants, ExperimentalMusclesRats, TransgenicRegenerationTissue EngineeringGreen Fluorescent Proteinscell migrationcell trackingtdTomatotissue engineering

Identifiers

PMID29490563
PMCPMC5946766
OpenAlexW2793772679

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.