Evidence map›Paper›PMID 29488127›Full record

ArticleMolecular biotechnology2018

Combined Strategies to Improve the Expression of Recombinant Sterol C24-Methyltransferase from Leishmania braziliensis in E. coli.

Humberto F Freitas, Acássia Benjamim Leal Pires, Marcelo S Castilho

Abstract read
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In one paragraph

Article in Molecular biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Humberto F FreitasFaculdade de Farmácia, Universidade Federal da Bahia, Salvador, BA, 40170-290, Brazil.
Acássia Benjamim Leal PiresDepartamento de Ciências da Vida, Universidade do Estado da Bahia, Salvador, BA, 41150-000, Brazil.
Marcelo S CastilhoFaculdade de Farmácia, Universidade Federal da Bahia, Salvador, BA, 40170-290, Brazil. castilho@ufba.br.
Universidade Estadual de Feira de Santana · BRUniversidade Salvador · BR

Funding

Fundação de Amparo à Pesquisa do Estado da Bahia 4903/2012
6 · The paper itself

Abstract

Among the neglected tropical diseases, leishmaniasis stands out for its worldwide distribution and diversity of symptoms. Cutaneous leishmaniasis (CL), for instance, is endemic in 18 countries, but the available drugs to fight it have high toxicity and low patient adherence. In order to overcome this, dilemma drugs that target enzymes which are absent in the human host, such as Leishmania braziliensis sterol C24-methyltransferase (SMT-C24, EC 2.1.1.41), are needed. However, medicinal chemistry efforts toward this goal have been hampered by the low yield of soluble recombinant SMT-C24 afforded by currently available expression systems. Herein, we show that a combination of molecular biology and chromatographic strategies may increase the yield of LbSMT-C24 in up to fivefold. These results lay the ground for future investigation of this enzyme as a drug target.

Indexed as

Cloning, MolecularEscherichia coliLeishmania braziliensisMethyltransferasesProtein EngineeringProtozoan ProteinsRecombinant ProteinsMethyltransferasesProtozoan ProteinsRecombinant ProteinsHeterologous expressionLeishmania braziliensisSterol C24-methyltransferase

Identifiers

PMID29488127
OpenAlexW2794405844

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.