ArticleNeurophotonics2018
Designing a large field-of-view two-photon microscope using optical invariant analysis.
Article in Neurophotonics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 85 citations in OpenAlex.
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- Ultra-wide-field, deep, adaptive two-photon microscopy for multi-scale neuronal imaging.Light, science & applications · 2026Article
- Long-range axon branching: contributions to brain network plasticity and repair.Nature reviews. Neuroscience · 2026Review
- Cellular multipoint adaptive technology for two-photon mesoscope.Neurophotonics · 2026Article
- Simultaneous thermal and large field-of-view two-photon imaging enables optimization of signal-to-noise.Neurophotonics · 2026Article
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- Standardized measurements for monitoring and comparing multiphoton microscope systems.Nature protocols · 2025Review
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Non-inertial scan angle multiplier for expanded fields-of-view.bioRxiv : the preprint server for biology · 2025Article
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- Compact module for video-rate image mosaics in two-photon microscopy.Optics express · 2025Article
- TWINKLE: An open-source two-photon microscope for teaching and research.bioRxiv : the preprint server for biology · 2025Article
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- Direct laser writing-enabled 3D printing strategies for microfluidic applications.Lab on a chip · 2024Review
- Two-dimensional electro-optical multiphoton microscopy.Neurophotonics · 2024Article
- Comprehensive software suite for functional analysis and synaptic input mapping of dendritic spines imagedNeurophotonics · 2024Article
- Longitudinal intravital imaging of mouse placenta.Science advances · 2024Article
- Standardised Measurements for Monitoring and Comparing Multiphoton Microscope Systems.bioRxiv : the preprint server for biology · 2024Article
- Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity.Nature communications · 2023Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Conventional two-photon microscopy (TPM) is capable of imaging neural dynamics with subcellular resolution, but it is limited to a field-of-view (FOV) diameter [Formula: see text]. Although there has been recent progress in extending the FOV in TPM, a principled design approach for developing large FOV TPM (LF-TPM) with off-the-shelf components has yet to be established. Therefore, we present a design strategy that depends on analyzing the optical invariant of commercially available objectives, relay lenses, mirror scanners, and emission collection systems in isolation. Components are then selected to maximize the space-bandwidth product of the integrated microscope. In comparison with other LF-TPM systems, our strategy simplifies the sequence of design decisions and is applicable to extending the FOV in any microscope with an optical relay. The microscope we constructed with this design approach can image [Formula: see text] lateral and [Formula: see text] axial resolution over a 7-mm diameter FOV, which is a 100-fold increase in FOV compared with conventional TPM. As a demonstration of the potential that LF-TPM has on understanding the microarchitecture of the mouse brain across interhemispheric regions, we performed
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.