ArticleFrontiers in microbiology2018
Highly Sensitive Colorimetric Biosensor for Staphylococcal Enterotoxin B by a Label-Free Aptamer and Gold Nanoparticles.
Article in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 86 citations in OpenAlex.
- An overview of CRISPR-artificial intelligence theranostics: Current and emerging applications.Biomaterials translational · 2026Review
- Gold nanoparticle-based colorimetric detection of ATP via a label-free aptamer.Frontiers in bioengineering and biotechnology · 2026Article
- Nanomaterials for bacterial enrichment and detection in healthcare.Nanomedicine (London, England) · 2025Review
- Sensing the Future-Frontiers in Biosensors: Exploring Classifications, Principles, and Recent Advances.ACS omega · 2024Review
- Advances in Aptamer-Based Biosensors for the Detection of Foodborne Mycotoxins.Molecules (Basel, Switzerland) · 2024Review
- Recent Advances in Aptamer-Based Biosensors for Bacterial Detection.Biosensors · 2024Review
- Screening biotoxin aptamer and their application of optical aptasensor in food stuff: a review.Frontiers in chemistry · 2024Review
- Article
- Conventional and advanced detection techniques of foodborne pathogens: A comprehensive review.Heliyon · 2023Article
- Highly Specific Detection of Oxytocin in Saliva.International journal of molecular sciences · 2023Article
- An aptasensor for ampicillin detection in milk by fluorescence resonance energy transfer between upconversion nanoparticles and Au nanoparticles.Food chemistry: X · 2022Article
- Progress and Challenges of Point-of-Need Photonic Biosensors for the Diagnosis of COVID-19 Infections and Immunity.Biosensors · 2022Review
- Combating small molecule environmental contaminants: detection and sequestration using functional nucleic acids.Chemical science · 2022Review
- Article
- Optimisation of a gold nanoparticle-based aptasensor integrated with image processing for the colorimetric detection of acephate using response surface methodology.RSC advances · 2021Article
- Recent progress in the optical detection of pathogenic bacteria based on noble metal nanoparticles.Mikrochimica acta · 2021Review
- Recent Advances in Nanomaterial-Based Aptasensors in Medical Diagnosis and Therapy.Nanomaterials (Basel, Switzerland) · 2021Review
- Recent advances on aptamer-based biosensors for detection of pathogenic bacteria.World journal of microbiology & biotechnology · 2021Review
- Functionalized gold nanoparticles: promising and efficient diagnostic and therapeutic tools for HIV/AIDS.RSC medicinal chemistry · 2020Review
- Development of a biosensor from aptamers for detection of the porcine reproductive and respiratory syndrome virus.Journal of veterinary science · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple, sensitive and selective colorimetric biosensor for the detection of Staphylococcal enterotoxin B (SEB) was developed using SEB-binding aptamer (SEB2) as recognition element and unmodified gold nanoparticles (AuNPs) as colorimetric probes. The assay is based on color change from red to purple due to conformational change of aptamer in the presence of SEB, and the phenomenon of salt-induced AuNPs aggregation which could be monitored by naked eye or UV-vis spectrometer. Results showed that the AuNPs can effectively differentiate the SEB induced conformational change of the aptamer in the presence of a given high salt concentration. A linear response in the range of 50 μg/mL to 0.5 ng/mL of SEB concentration was obtained. The assay was highly specific to SEB as compared to other related toxins. The limit of detection (LOD) of SEB achieved within few minutes was 50 ng/mL visually and spectrometric method improved it to 0.5 ng/mL. Robustness of the assay was tested in artificially spiked milk samples and cross-checked using in house developed sandwich ELISA (IgY as capturing and SEB specific monoclonal as revealing antibody) and PCR. This colorimetric assay could be a suitable alternative over existing methods during biological emergencies due to its simplicity, sensitive and cost effectiveness.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.