ArticleScientific reports2018
Improving RNAi in the Brown Marmorated Stink Bug: Identification of target genes and reference genes for RT-qPCR.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 75 citations in OpenAlex.
- Identification of broadly expressed nervous system-related genes as effective RNAi targets in the brown marmorated stink bug, Halyomorpha halys.Pest management science · 2026Article
- Oral RNAi in Euschistus heros: Evaluation of Gene Silencing Using Cationic Polymer-Stabilized dsRNA.Neotropical entomology · 2026Article
- Review
- Improving RNAi in the fall armyworm,Frontiers in insect science · 2026Article
- Control of Aedes albopictus populations by silencing of the vesicular GABA transporter (vgat) and the vesicular monoamine transporter (vmat) genes using recombinant Chlorella shRNA.Parasites & vectors · 2025Article
- Comparative analysis of convergent and divergent T7 RNA polymerase promoters for the synthesis of dsRNA in vivo and in vitro.RNA (New York, N.Y.) · 2025Article
- Double-Stranded DNA Reduces dsRNA Degradation in the Saliva and Significantly Enhanced RNAi-Mediated Gene Silencing in Halyomorpha halys.Advanced biology · 2025Article
- Article
- Assessment of housekeeping genes stability for gene transcription regulation analysis of Spodoptera littoralis (Lepidoptera: Noctuidae) under Spodoptera littoralis nucleopolyhedrovirus viral infection.Molecular biology reports · 2024Article
- Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in the Bean Bug,Insects · 2023Article
- Differential responses to double-stranded RNA injection and feeding in Mormon cricket (Orthoptera: Tettigoniidae).Journal of insect science (Online) · 2023Article
- Nanoparticle-Shielded dsRNA Delivery for Enhancing RNAi Efficiency in Cotton Spotted BollwormInternational journal of molecular sciences · 2023Article
- RNAi turns 25:contributions and challenges in insect science.Frontiers in insect science · 2023Review
- Trunk injection delivery of dsRNA for RNAi-based pest control in apple trees.Pest management science · 2022Article
- RNAi Efficiency through dsRNA Injection Is Enhanced by Knockdown of dsRNA Nucleases in the Fall Webworm,International journal of molecular sciences · 2022Article
- Plastid Transformation of Micro-Tom Tomato with a Hemipteran Double-Stranded RNA Results in RNA Interference in Multiple Insect Species.International journal of molecular sciences · 2022Article
- Dynamics in Pest Status of Phytophagous Stink Bugs in the Neotropics.Neotropical entomology · 2022Review
- RNAi inFrontiers in plant science · 2022Article
- Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide.Frontiers in zoology · 2021Review
- Tripartite parasitic and symbiotic interactions as a possible mechanism of horizontal gene transfer.Ecology and evolution · 2021Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The brown marmorated stink bug (BMSB) is native to Asia and recently invaded the USA. RNA interference (RNAi) is a gene silencing mechanism in which the introduction of double-stranded RNA (dsRNA) inhibits gene function by degrading target mRNA. In dsRNA stability assays, the dsRNases present in the hemolymph and salivary gland secretions of BMSB showed lower activity than those in the hemolymph of Heliothis virescens. We evaluated six housekeeping genes (18S rRNA, EF1-α, Actin, Ubiquitin, 60S RP and β-Tubulin) across dsRNA treatments (injection and feeding) in nymphs and adults of BMSB and identified 18S rRNA and 60S RP as the best genes to use as a reference in reverse-transcriptase quantitative PCR (RT-qPCR). Homologs of 13 genes that were shown to function as effective RNAi targets in other insects were identified and evaluated by injecting dsRNA targeting these homologs into BMSB adults. Five out of 13 dsRNAs tested caused more than 70% mortality by seven days after injection of dsRNA. Feeding dsRNA targeting five of these genes (IAP, ATPase, SNF7, GPCR, and PPI) to nymphs caused more than 70% mortality by three of the five dsRNAs tested. These data suggest that feeding dsRNA causes target gene knockdown and mortality in BMSB.
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