ArticleProceedings of the National Academy of Sciences of the United States of America2018
Human Ska complex and Ndc80 complex interact to form a load-bearing assembly that strengthens kinetochore-microtubule attachments.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Microtubule-Associated Proteins: From Dynamic Regulation of Microtubules to Cellular Architecture.Cells · 2026Review
- Kinetochore-microtubule attachments are strengthened by Cnn1 stabilization of Stu2.bioRxiv : the preprint server for biology · 2026Article
- Structural Basis of Human Kinetochore-Microtubule Coupling by the Ndc80 and Ska Complexes.bioRxiv : the preprint server for biology · 2026Article
- A modular chromosomal passenger complex rewires chromosome segregation in Plasmodium berghei.Nature communications · 2026Article
- Functional Interaction of SKA and NDC80 Complexes at Kinetochores Promoting Anaphase Onset in Mitosis.bioRxiv : the preprint server for biology · 2026Article
- Assembly and phosphoregulatory mechanisms of the budding yeast outer kinetochore KMN complex.The Journal of cell biology · 2026Article
- Microtubule end stabilisation by cooperative oligomers of Ska and Ndc80 complexes.The EMBO journal · 2026Article
- Molecular basis of cooperative assembly of the Ndc80-Ska kinetochore complex on microtubules.bioRxiv : the preprint server for biology · 2026Article
- SKAP binding to microtubules reduces friction at the kinetochore-microtubule interface and increases attachment stability under force.Current biology : CB · 2025Article
- SKA1 promotes oncogenic properties in oral dysplasia and oral squamous cell carcinoma, and augments resistance to radiotherapy.Molecular oncology · 2025Article
- Kinetochores grip microtubules with directionally asymmetric strength.The Journal of cell biology · 2025Article
- Analysis of a cancer-associated mutation in the budding yeast Nuf2 kinetochore protein.microPublication biology · 2025Article
- DNA packaging by molecular motors: from bacteriophage to human chromosomes.Nature reviews. Genetics · 2024Review
- SKAP binding to microtubules reduces friction at the kinetochore-microtubule interface and increases attachment stability under force.bioRxiv : the preprint server for biology · 2024Article
- A conserved site on Ndc80 complex facilitates dynamic recruitment of Mps1 to yeast kinetochores to promote accurate chromosome segregation.Current biology : CB · 2024Article
- SKA3 Expression as a Prognostic Factor for Patients with Pancreatic Adenocarcinoma.International journal of molecular sciences · 2024Article
- Article
- A coadapted KNL1 and spindle assembly checkpoint axis orchestrates precise mitosis in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Higher-order protein assembly controls kinetochore formation.Nature cell biology · 2024Article
2 more citing papers are in PubMed but not listed here.
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6 authors.
Funding
Abstract
Accurate segregation of chromosomes relies on the force-bearing capabilities of the kinetochore to robustly attach chromosomes to dynamic microtubule tips. The human Ska complex and Ndc80 complex are outer-kinetochore components that bind microtubules and are required to fully stabilize kinetochore-microtubule attachments in vivo. While purified Ska complex tracks with disassembling microtubule tips, it remains unclear whether the Ska complex-microtubule interaction is sufficiently strong to make a significant contribution to kinetochore-microtubule coupling. Alternatively, Ska complex might affect kinetochore coupling indirectly, through recruitment of phosphoregulatory factors. Using optical tweezers, we show that the Ska complex itself bears load on microtubule tips, strengthens Ndc80 complex-based tip attachments, and increases the switching dynamics of the attached microtubule tips. Cross-linking mass spectrometry suggests the Ska complex directly binds Ndc80 complex through interactions between the Ska3 unstructured C-terminal region and the coiled-coil regions of each Ndc80 complex subunit. Deletion of the Ska complex microtubule-binding domain or the Ska3 C terminus prevents Ska complex from strengthening Ndc80 complex-based attachments. Together, our results indicate that the Ska complex can directly strengthen the kinetochore-microtubule interface and regulate microtubule tip dynamics by forming an additional connection between the Ndc80 complex and the microtubule.
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