ArticleThe Journal of biological chemistry2018
Hypoxanthine is a checkpoint stress metabolite in colonic epithelial energy modulation and barrier function.
Article in The Journal of biological chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
93 citing papers in PubMed, 149 citations in OpenAlex.
- Baseline Serum and Stool Microbiome Biomarkers Predict Clinical Efficacy and Tissue Molecular Response After Ritlecitinib Induction Therapy in Ulcerative Colitis.Journal of Crohn's & colitis · 2024Trial
- Article
- Perfluorooctanoic acid exposure disrupts gut microbiota and aggravates experimental colitis.Environmental pollution (Barking, Essex : 1987) · 2026Article
- Enterochromaffin cells as a cellular integration hub for cooperative microbial signaling to modulate gut serotonin and motility.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- CARM1-mediated hypoxanthine-enriched exosomes rewire inosine metabolism and impair CD8Cell death and differentiation · 2026Article
- Epidemiological and Metabolic Characteristics ofAnimals : an open access journal from MDPI · 2026Article
- Metabolic host-microbe crosstalk in stabilization of epithelial HIF.Microbiome · 2026Article
- AMPK Deficiency Induces Corneal Epithelial Barrier Dysfunction by Modulating Energy Homeostasis.Investigative ophthalmology & visual science · 2026Article
- Preksha Dhyana meditation modulates the serum metabolome in healthy and meditation-naïve participants.Frontiers in molecular biosciences · 2026Article
- Modifying microbially derived short chain fatty acids to promote health.The Journal of physiology · 2026Review
- Susceptibility to mild and severe acute mountain sickness is associated with distinct urine metabolite profiles.Frontiers in physiology · 2026Article
- Article
- Article
- A scalable long-termbioRxiv : the preprint server for biology · 2025Article
- DNA-utilization loci enable exogenous DNA metabolism in gut Bacteroidales.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Untargeted metabolomics as a tool to assess the impact of dietary approaches on pig gut health: a review.Journal of animal science and biotechnology · 2025Review
- An Integrative Approach Using Molecular and Metabolomic Studies Reveals the Connection of Glutamic Acid with Telomerase and Oxidative Stress in Berberine-Treated Colorectal Cancer Cell Line HCT 116.Applied biochemistry and biotechnology · 2025Article
- Gut microbiota and relevant metabolites analysis in perianal abscess of infants.BMC microbiology · 2025Article
- A Novel Supramolecular Salt of Hypoxanthine with Maleic Acid as a Potential Weight-Loss Drug.International journal of molecular sciences · 2025Article
- Diet-induced changes in metabolism influence immune response and viral shedding in Jamaican fruit bats.Proceedings. Biological sciences · 2025Article
33 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Intestinal epithelial cells form a selectively permeable barrier to protect colon tissues from luminal microbiota and antigens and to mediate nutrient, fluid, and waste flux in the intestinal tract. Dysregulation of the epithelial cell barrier coincides with profound shifts in metabolic energy, especially in the colon, which exists in an energetically depleting state of physiological hypoxia. However, studies that systematically examine energy flux and adenylate metabolism during intestinal epithelial barrier development and restoration after disruption are lacking. Here, to delineate barrier-related energy flux, we developed an HPLC-based profiling method to track changes in energy flux and adenylate metabolites during barrier development and restoration. Cultured epithelia exhibited pooling of phosphocreatine and maintained ATP during barrier development. EDTA-induced epithelial barrier disruption revealed that hypoxanthine levels correlated with barrier resistance. Further studies uncovered that hypoxanthine supplementation improves barrier function and wound healing and that hypoxanthine appears to do so by increasing intracellular ATP, which improved cytoskeletal G- to F-actin polymerization. Hypoxanthine supplementation increased the adenylate energy charge in the murine colon, indicating potential to regulate adenylate energy charge-mediated metabolism in intestinal epithelial cells. Moreover, experiments in a murine colitis model disclosed that hypoxanthine loss during active inflammation correlates with markers of disease severity. In summary, our results indicate that hypoxanthine modulates energy metabolism in intestinal epithelial cells and is critical for intestinal barrier function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.