Evidence map›Paper›PMID 29487088›Full record

ArticleThe Journal of general physiology2018

Reconstitution and functional characterization of ion channels from nanodiscs in lipid bilayers.

Laura-Marie Winterstein, Kerri Kukovetz, Oliver Rauh, Daniel L Turman, Christian Braun, Anna Moroni, Indra Schroeder, Gerhard Thiel

Abstract read
In one paragraph

Article in The Journal of general physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed.

  1. Similarity of the ion occupations of KChannels (Austin, Tex.) · 2026
    Article
  2. Article
  3. Conformational landscape and clustering of human type 2 IPbioRxiv : the preprint server for biology · 2026
    Article
  4. Article
  5. Perturbation of the host cell CaCell death & disease · 2023
    Article
  6. Review
  7. Review
  8. Article
  9. Review
  10. Article
  11. Article
  12. Asymmetric Interplay Between KFrontiers in physiology · 2021
    Article
  13. Review
  14. Article
  15. A Functional KViruses · 2020
    Article
  16. Article
  17. Cell-Free Protein Synthesis: A Promising Option for Future Drug Development.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2020
    Review
  18. Cryo-EM structures of a lipid-sensitive pentameric ligand-gated ion channel embedded in a phosphatidylcholine-only bilayer.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Laura-Marie WintersteinPlant Membrane Biophysics, Technische Universität Darmstadt, Darmstadt, Germany.ORCID 0000-0001-9482-8164
Kerri KukovetzPlant Membrane Biophysics, Technische Universität Darmstadt, Darmstadt, Germany.
Oliver RauhPlant Membrane Biophysics, Technische Universität Darmstadt, Darmstadt, Germany.ORCID 0000-0003-1082-8656
Daniel L TurmanDepartment of Biochemistry and Howard Hughes Medical Institute, Brandeis University, Waltham, MA.ORCID 0000-0003-2965-5484
Christian BraunPlant Membrane Biophysics, Technische Universität Darmstadt, Darmstadt, Germany.
Anna MoroniDepartment of Biosciences and Consiglio Nazionale delle Ricerche - Istituto di Biofisica, Università degli Studi di Milano, Milano, Italy.
Indra SchroederPlant Membrane Biophysics, Technische Universität Darmstadt, Darmstadt, Germany.ORCID 0000-0002-7875-983X
Gerhard ThielPlant Membrane Biophysics, Technische Universität Darmstadt, Darmstadt, Germany thiel@bio.tu-darmstadt.de.ORCID 0000-0002-2335-1351

Funding

Macromolecular Structure MechanismT32GM007596 · NIGMS · BRANDEIS UNIVERSITY · PI OPRIAN, DANIEL D · 1985 to 2019
$4.5M
Mechanisms of Biological Fluoride Resistance ExportersR01GM107023 · NIGMS · BRANDEIS UNIVERSITY · PI MILLER, CHRISTOPHER · 2014 to 2017
$1.1M
European Research Council 695078Howard Hughes Medical InstituteNIGMS NIH HHS R01 GM107023NIGMS NIH HHS T32 GM007596
6 · The paper itself

Abstract

Recent studies have shown that membrane proteins can be efficiently synthesized in vitro before spontaneously inserting into soluble nanoscale lipid bilayers called nanodiscs (NDs). In this paper, we present experimental details that allow a combination of in vitro translation of ion channels into commercially available NDs followed by their direct reconstitution from these nanobilayers into standard bilayer setups for electrophysiological characterization. We present data showing that two model K

Indexed as

Action PotentialsBacterial ProteinsLipid BilayersPotassium ChannelsViral ProteinsBacterial ProteinsKcv potassium channel, Chlorella virusLipid BilayersPotassium Channelsprokaryotic potassium channelViral Proteins

Identifiers

PMID29487088
PMCPMC5881443

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.