ArticleParasites & vectors2018
Blood-feeding, susceptibility to infection with Schmallenberg virus and phylogenetics of Culicoides (Diptera: Ceratopogonidae) from the United Kingdom.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- The changing landscape of bluetongue in northern Europe.Journal of medical entomology · 2026Review
- The Role of Hematophagous Arthropods, Other than Mosquitoes and Ticks, in Arbovirus Transmission.Viruses · 2025Review
- Schmallenberg virus non-structural proteins NSs and NSm are not essential for experimental infection ofJournal of virology · 2025Article
- Modelling the Influence of Climate and Vector Control Interventions on Arbovirus Transmission.Viruses · 2024Article
- Occurrence of putative Culicoides biting midge vectors (Diptera: Ceratopogonidae) inside and outside barns in Germany and factors influencing their activity.Parasites & vectors · 2023Article
- A Review of the Vector Status of North AmericanCurrent tropical medicine reports · 2022Review
- A study of the composition of the Obsoletus complex and genetic diversity of Culicoides obsoletus populations in Spain.Parasites & vectors · 2021Article
- Species composition and relative abundance of the genus Culicoides (Diptera: Ceratopogonidae) in Romania.Parasites & vectors · 2020Article
- A phylogenetic analysis of the biting midges belonging to Culicoides Latreille (Diptera: Ceratopogonidae) subgenus Avaritia using molecular data.Parasites & vectors · 2020Article
- Blood-meal analysis of Culicoides (Diptera: Ceratopogonidae) reveals a broad host range and new species records for Romania.Parasites & vectors · 2020Article
- Endemic and Emerging Arboviruses in Domestic Ruminants in East Asia.Frontiers in veterinary science · 2020Review
- Vector Competence of Italian Populations ofViruses · 2019Article
- Vector competence of biting midges and mosquitoes for Shuni virus.PLoS neglected tropical diseases · 2019Article
- Schmallenberg virus: a systematic international literature review (2011-2019) from an Irish perspective.Irish veterinary journal · 2019Review
- Vector competence of biting midges and mosquitoes for Shuni virus.PLoS neglected tropical diseases · 2018Article
- Vector competence of pre-alpine Culicoides (Diptera: Ceratopogonidae) for bluetongue virus serotypes 1, 4 and 8.Parasites & vectors · 2018Article
- Reproducibility and relevance in insect-arbovirus infection studies.Current opinion in insect science · 2018Review
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
backgroundCulicoides biting midges (Diptera: Ceratopogonidae) are responsible for the biological transmission of internationally important arboviruses of livestock. In 2011, a novel Orthobunyavirus was discovered in northern Europe causing congenital malformations and abortions in ruminants. From field studies, Culicoides were implicated in the transmission of this virus which was subsequently named Schmallenberg virus (SBV), but to date no assessment of susceptibility to infection of field populations under standardised laboratory conditions has been carried out. We assessed the influence of membrane type (chick skin, collagen, Parafilm M®) when offered in conjunction with an artificial blood-feeding system (Hemotek, UK) on field-collected Culicoides blood-feeding rates. Susceptibility to infection with SBV following blood-feeding on an SBV-blood suspension provided via either (i) the Hemotek system or via (ii) a saturated cotton wool pledglet was then compared. Schmallenberg virus susceptibility was defined by RT-qPCR of RNA extractions of head homogenates and related to Culicoides species and haplotype identifications based on the DNA barcode region of the mitochondrial cytochrome c oxidase 1 (cox1) gene.
resultsCulicoides blood-feeding rates were low across all membrane types tested (7.5% chick skin, 0.0% for collagen, 4.4% Parafilm M®, with 6029 female Culicoides being offered a blood meal in total). Susceptibility to infection with SBV through membrane blood-feeding (8 of 109 individuals tested) and pledglet blood-feeding (1 of 94 individuals tested) was demonstrated for the Obsoletus complex, with both C. obsoletus (Meigen) and C. scoticus Downes & Kettle susceptible to infection with SBV through oral feeding. Potential evidence of cryptic species within UK populations was found for the Obsoletus complex in phylogenetic analyses of cox1 DNA barcodes of 74 individuals assessed from a single field-site.
conclusionsMethods described in this study provide the means to blood-feed Palaearctic Culicoides for vector competence studies and colonisation attempts. Susceptibility to SBV infection was 7.3% for membrane-fed members of the subgenus Avaritia and 1.1% for pledglet-fed. Both C. obsoletus and C. scoticus were confirmed as being susceptible to infection with SBV, with potential evidence of cryptic species within UK Obsoletus complex specimens, however the implications of cryptic diversity in the Obsoletus complex on arbovirus transmission remains unknown.
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