ArticleAntioxidants & redox signaling2019
Single-Cell Transcriptomics of Human Oocytes: Environment-Driven Metabolic Competition and Compensatory Mechanisms During Oocyte Maturation.
Article in Antioxidants & redox signaling, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.
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Who cites it
30 citing papers in PubMed, 2 syntheses or guidelines pooled it, 65 citations in OpenAlex.
- Systematic review of mRNA expression in human oocytes: understanding the molecular mechanisms underlying oocyte competence.Journal of assisted reproduction and genetics · 2023Pooled it
- Endoplasmic reticulum in oocytes: spatiotemporal distribution and function.Journal of assisted reproduction and genetics · 2023Pooled it
- Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses.Journal of ovarian research · 2022Trial
- Metabolomic profiling delineates the role of adenosine in oocyte quality and embryonic development.Cell death & disease · 2026Article
- Melatonin as a Potential Dietary Supplement to Counteract Glyphosate-Induced Decline in Honeybee Populations.Insects · 2026Article
- Transcriptomics empower assisted reproductive technology in decoding gamete and embryo development potential: a review.Journal of assisted reproduction and genetics · 2025Review
- Single-cell multiomic analysis reveals methylome and transcriptome deviations following oocyte maturation in vitro.Reproduction (Cambridge, England) · 2025Article
- Rapamycin reduces DNA damage of in vitro matured oocytes by promoting Rad51-mediated homologous recombination.Reproductive biology and endocrinology : RB&E · 2025Article
- Transcriptional insights on the incomplete cytoplasmic maturation and developmental potential of oocytes cultured without granulosa cells in mice.BMC genomics · 2025Article
- Current status and hotspots of in vitro oocyte maturation: a bibliometric study of the past two decades.Journal of assisted reproduction and genetics · 2025Review
- Spatiotemporal Distribution and Function of Mitochondria in Oocytes.Reproductive sciences (Thousand Oaks, Calif.) · 2024Review
- Application of Single-Cell RNA Sequencing in Ovarian Development.Biomolecules · 2022Review
- Single-cell RNA-sequencing of retrieved human oocytes and eggs in clinical practice and for human ovarian cell atlasing.Molecular reproduction and development · 2022Review
- Rapamycin improves the quality and developmental competence of mice oocytes by promoting DNA damage repair during in vitro maturation.Reproductive biology and endocrinology : RB&E · 2022Article
- Single-Cell Sequencing Reveals an Intrinsic Heterogeneity of the Preovulatory Follicular Microenvironment.Biomolecules · 2022Article
- Rapamycin improves the developmental competence of human oocytes by alleviating DNA damage during IVM.Human reproduction open · 2022Article
- Article
- GVBD rate is an independent predictor for pregnancy in ICSI patients with surplus immature oocytes.Frontiers in endocrinology · 2022Article
- Inhibiting bridge integrator 2 phosphorylation leads to improved oocyte quality, ovarian health and fertility in aging and after chemotherapy in mice.Nature aging · 2021Article
- The Role of Mitochondria in Oocyte Maturation.Cells · 2021Review
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsThe mechanisms coordinating maturation with an environment-driven metabolic shift, a critical step in determining the developmental potential of human in vitro maturation (IVM) oocytes, remain to be elucidated. Here we explored the key genes regulating human oocyte maturation using single-cell RNA sequencing and illuminated the compensatory mechanism from a metabolic perspective by analyzing gene expression.
resultsThree key genes that encode CoA-related enzymes were screened from the RNA sequencing data. Two of them, ACAT1 and HADHA, were closely related to the regulation of substrate production in the Krebs cycle. Dysfunction of the Krebs cycle was induced by decreases in the activity of specific enzymes. Furthermore, the activator of these enzymes, the calcium concentration, was also decreased because of the failure of influx of exogenous calcium. Although release of endogenous calcium from the endoplasmic reticulum and mitochondria met the requirement for maturation, excessive release resulted in aneuploidy and developmental incompetence. High nicotinamide nucleotide transhydrogenase expression induced NADPH dehydrogenation to compensate for the NADH shortage resulting from the dysfunction of the Krebs cycle. Importantly, high NADP INNOVATION: The present study shows for the first time that exposure to the in vitro environment can lead to the decline of energy metabolism in human oocytes during maturation but that a compensatory action maintains their developmental competence.
conclusionIn vitro maturation of human oocytes is mediated through a cascade of competing and compensatory actions driven by genes encoding enzymes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.