ArticleIntegrative cancer therapies2018
Chaga ( Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B).
Article in Integrative cancer therapies, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 76 citations in OpenAlex.
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- Current Knowledge and Utilization of Medicinal Plants and Fungi in Northeastern Croatia.Plants (Basel, Switzerland) · 2026Article
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- Macromycete Edible Fungi as a Functional Poultry Feed Additive: Influence on Health, Welfare, Eggs, and Meat Quality-Review.Molecules (Basel, Switzerland) · 2025Review
- INO10, a Chaga Mushroom Extract, Alleviates Alzheimer's Disease-Related Pathology and Cognitive Deficits in 3xTg-AD Mice.International journal of molecular sciences · 2025Article
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- Chemical Content and Cytotoxic Activity on Various Cancer Cell Lines of Chaga (Pharmaceuticals (Basel, Switzerland) · 2024Article
- A review on the cultivation, bioactive compounds, health-promoting factors and clinical trials of medicinal mushrooms Taiwanofungus camphoratus, Inonotus obliquus and Tropicoporus linteus.Fungal biology and biotechnology · 2024Review
- Comparative Cytotoxicity Evaluation of Heat-AssistedFood technology and biotechnology · 2024Article
- The Effect of Green Extraction Technologies on the Chemical Composition of Medicinal Chaga Mushroom Extracts.Journal of fungi (Basel, Switzerland) · 2024Article
- Article
- From Nature to Design: Tailoring Pure Mycelial Materials for the Needs of Tomorrow.Journal of fungi (Basel, Switzerland) · 2024Review
- Synergistic Cytotoxicity of Extracts of Chaga Mushroom and Microalgae against Mammalian Cancer Cells In Vitro.Oxidative medicine and cellular longevity · 2024Article
- Preventive effects of inotodiol on polyinosinic-polycytidylic acid-induced inflammation in human dermal fibroblasts.Heliyon · 2023Article
- Medicinal Mushrooms: Their Bioactive Components, Nutritional Value and Application in Functional Food Production-A Review.Molecules (Basel, Switzerland) · 2023Review
- Comparison of Bioactive Secondary Metabolites and Cytotoxicity of Extracts fromMolecules (Basel, Switzerland) · 2023Article
- Investigating the Protective Role of Biochaga Drug on Structural Changes of Bovine Serum Albumin in the Presence of Methyl tert-butyl Ether.The protein journal · 2023Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInonotus obliquus, also known as Chaga, is a parasitic fungus growing on birches and used in traditional medicine (especially by Khanty people) to treat various health problems. In this study, we aimed to quantify the 3 metabolites frequently cited in literature, that is, betulin, betulinic acid, and inotodiol in the Chaga recently discovered in forests located in Normandy (France), and to compare their concentrations with Ukrainian and Canadian Chaga. This study also explores the cytotoxicity of the French Chaga against cancer-derived cells and transformed cells.
methodsA quantification method by HPLC-MS-MS (high-performance liquid chromatography-tandem mass spectrometry) of betulin, betulinic acid, and inotodiol was developed to study the French Chaga and compare the concentration of these metabolites with extracts provided from Chaga growing in Canada and Ukraine. This method was also used to identify and quantify those 3 compounds in other traditional preparations of Chaga (aqueous extract, infusion, and decoction). Among these preparations, the aqueous extract that contains betulin, betulinic acid, and inotodiol was chosen to evaluate and compare its cytotoxic activity toward human lung adenocarcinoma cells (A549 line) and human bronchial epithelial cells (BEAS-2B line).
resultsFrench Chaga contains betulin and betulinic acid at higher levels than in other Chaga, whereas the concentration of inotodiol is greater in the Canadian Chaga. Moreover, the results highlighted a cytotoxic activity of the Chaga's aqueous extract after 48 and 72 hours of exposure with a higher effect on cancer-derived cells A549 than on normal transformed cells BEAS-2B ( P = 0.025 after 48 hours of exposure and P = 0.004 after 72 hours of exposure).
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